验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, human colon tissue, HEK-293 cells, mouse kidney tissue, L02 cells, HepG2 cells |
| Positive IP detected in | HEK-293 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13549-1-AP targets NAPRT1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IP, ELISA Application Description |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | NAPRT1 fusion protein Ag4265 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | nicotinate phosphoribosyltransferase domain containing 1 |
| Synonyms | FHA HIT interacting protein, FHIP, NAPRT1, NAPRTase, PP3856 |
| Calculated Molecular Weight | 514 aa, 55 kDa |
| Observed Molecular Weight | 51 kDa, 56 kDa |
| GenBank Accession Number | BC032466 |
| Gene Symbol | NAPRT1 |
| Gene ID (NCBI) | 93100 |
| RRID | AB_2151014 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6XQN6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nicotinic acid (NA) is a coenzyme in cellular redox reactions, and is an essential component of metabolic pathways in all living cells. NAPRT1 (Nicotinate phosphoribosyltransferase) is essential for increasing cellular NAD levels and, thus, to prevent oxidative stress of cells. NAPRT1 converts Nicotinic acid (NA; niacin) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NAPRT1 antibody 13549-1-AP | Download protocol |
| IP protocol for NAPRT1 antibody 13549-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab NAD+ Metabolism Maintains Inducible PD-L1 Expression to Drive Tumor Immune Evasion. | ||
J Pharm Anal Epigenetic drug library screening reveals targeting DOT1L abrogates NAD+ synthesis by reprogramming H3K79 methylation in uveal melanoma | ||
Kidney Int Anticystogenic activity of a small molecule PAK4 inhibitor may be a novel treatment for autosomal dominant polycystic kidney disease. | ||
Clin Cancer Res Targeting of NAD metabolism in pancreatic cancer cells: potential novel therapy for pancreatic tumors. | ||
Int J Mol Sci NAD+ Anabolism Disturbance Causes Glomerular Mesangial Cell Injury in Diabetic Nephropathy. | ||
Metabolism Hyperglycemia-reduced NAD+ biosynthesis impairs corneal epithelial wound healing in diabetic mice. |
