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Napsin A Monoclonal antibody

Napsin A Monoclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1H7F2

Cat no : 60259-2-Ig

Print datasheet

Synonyms

NAPSA, KAP, EC:3.4.23.-, Aspartyl protease 4, ASP4

验证数据展示

  • IHC staining of human lung cancer using 60259-2-Ig

    IHC staining of human lung cancer using 60259-2-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60259-2-Ig (NAPSA antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Napsin A antibody included
  • IHC staining of human lung cancer using 60259-2-Ig

    IHC staining of human lung cancer using 60259-2-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60259-2-Ig (NAPSA antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Napsin A antibody included
  • FC experiment of A549 using 60259-2-Ig

    FC experiment of A549 using 60259-2-Ig

    1X10^6 A549 cells were intracellularly stained with 0.2 ug Anti-Human Napsin A (60259-2-Ig, Clone:1H7F2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HUVEC using 60259-2-Ig

    IF Staining of HUVEC using 60259-2-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using Napsin A antibody (60259-2-Ig, Clone: 1H7F2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive IHC detected inhuman lung cancer tissue, human kidney tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHUVEC cells
Positive FC (Intra) detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy Napsin A Ready-To-Use IHC Kit. Napsin A primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:10000-1:60000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

60259-2-Ig targets Napsin A in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen Napsin A fusion protein Ag9721 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name napsin A aspartic peptidase
Synonyms NAPSA, KAP, EC:3.4.23.-, Aspartyl protease 4, ASP4
Calculated Molecular Weight 420 aa, 45 kDa
GenBank Accession NumberBC017842
Gene Symbol Napsin A
Gene ID (NCBI) 9476
RRIDAB_2881381
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO96009
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Napsin is found in two isoforms, napsin A and B, with highly homologous nucleotide sequences (91.2%). Napsin A appears to be a functional proteinase, predominantly expressed in lung and kidney. Napsin B is transcribed exclusively in cells related to the immune system and lacks an in-frame stop codon and is believed to be a pseudogene.(PMID:12698189). Napsin A is superior to TTF-1 in distinguishing primary lung ACA from other carcinomas (except kidney), particularly primary lung small cell carcinoma, and primary thyroid carcinoma.(PMID:22288963).

实验方案

Product Specific Protocols
IHC protocol for Napsin A antibody 60259-2-IgDownload protocol
IF protocol for Napsin A antibody 60259-2-IgDownload protocol
FC protocol for Napsin A antibody 60259-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Nat Commun

Cancer cell-expressed BTNL2 facilitates tumour immune escape via engagement with IL-17A-producing γδ T cells.

Authors - Yanyun Du
View Article