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验证数据展示

  • WB analysis using 20209-1-AP

    WB analysis using 20209-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20209-1-AP (NARFL-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 20209-1-AP

    IHC staining of human kidney using 20209-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20209-1-AP (NARFL-Specific antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human kidney using 20209-1-AP

    IHC staining of human kidney using 20209-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20209-1-AP (NARFL-Specific antibody) at dilution of 1:100 (under 10x lens).

NARFL-Specific Polyclonal antibody

NARFL-Specific Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20209-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

CIAO3, HPRN, IOP1, LET1L, NARFL, NARFL-Specific, PRN, Protein related to Narf

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells
Positive IHC detected inhuman kidney tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

20209-1-AP targets NARFL-Specific in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear prelamin A recognition factor-like
别名 CIAO3, HPRN, IOP1, LET1L, NARFL, NARFL-Specific, PRN, Protein related to Narf
计算分子量 53 kDa
观测分子量 26-28 kDa
GenBank蛋白编号NM_022493
基因名称 NARFL
Gene ID (NCBI) 64428
RRIDAB_10694435
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H6Q4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NARFL, also named as PRN and IOP1, belongs to the NARF family. NAFL is a component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. It is required for maturation of extramitochondrial Fe/S proteins. It seems to negatively regulate the level of HIF1A expression, although this effect could be indirect. [18270200], [16956324] The antibody is specific to NARFL.

实验方案

Product Specific Protocols
WB protocol for NARFL-Specific antibody 20209-1-APDownload protocol
IHC protocol for NARFL-Specific antibody 20209-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Free Radic Biol Med

The iron-sulfur cluster assembly network component NARFL Is a key element in the cellular defense against oxidative stress.

Authors - Monique Corbin
View Article