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NARS Monoclonal antibody, PBS Only

NARS Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4G5G12

Cat no : 67711-1-PBS

Print datasheet

Synonyms

ASNRS, Asparagine tRNA ligase, asparaginyl tRNA synthetase, NARS, NARS1

验证数据展示

  • WB analysis using 67711-1-Ig (same clone as 67711-1-PBS)

    WB analysis using 67711-1-Ig (same clone as 67711-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67711-1-Ig (NARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67711-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67711-1-Ig (same clone as 67711-1-PBS)

    IF Staining of HeLa using 67711-1-Ig (same clone as 67711-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NARS antibody (67711-1-Ig, Clone: 4G5G12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67711-1-PBS in a different storage buffer formulation.

  • IHC staining of human urothelial carcinoma using 67711-1-Ig (same clone as 67711-1-PBS)

    IHC staining of human urothelial carcinoma using 67711-1-Ig (same clone as 67711-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67711-1-Ig (NARS antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67711-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NARS antibody included
Planning an IHC experiment? We recommend our IHCeasy NARS Ready-To-Use IHC Kit. NARS primary antibody included.

产品信息

67711-1-PBS targets NARS in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen NARS fusion protein Ag6656 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name asparaginyl-tRNA synthetase
Synonyms ASNRS, Asparagine tRNA ligase, asparaginyl tRNA synthetase, NARS, NARS1
Calculated Molecular Weight 63 kDa
Observed Molecular Weight63 kDa
GenBank Accession NumberBC001687
Gene Symbol NARS
Gene ID (NCBI) 4677
RRIDAB_2882901
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43776
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Asparagine-tRNA ligase (NARS) belongs to the class-II aminoacyl-tRNA synthetase family. Catalyzes the attachment of asparagine to tRNA(Asn) in a two-step reaction: asparagine is first activated by ATP to form Asn-AMP and then transferred to the acceptor end of tRNA(Asn) (PubMed: 9421509). In addition to its essential role in protein synthesis, acts as a signaling molecule that induced migration of CCR3-expressing cells. The NARS levels were higher in tumor tissues compared with adjacent normal tissues, and positively associated with LN metastasis. NARS may serve as a potential biomarker for lung adenocarcinoma (ADC) diagnosis/prognosis (PMID: 27161446).