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验证数据展示

  • WB analysis of A375 using 14394-1-AP

    WB analysis of A375 using 14394-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 14394-1-AP (NAT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 14394-1-AP

    IHC staining of human breast cancer using 14394-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14394-1-AP (NAT1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 14394-1-AP

    IHC staining of human breast cancer using 14394-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14394-1-AP (NAT1 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of A375 using 14394-1-AP

    IF Staining of A375 using 14394-1-AP

    Immunofluorescent analysis of A375 cells using 14394-1-AP (NAT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

NAT1 Polyclonal antibody

NAT1 Polyclonal Antibody for WB, IF, IHC, ELISA
Cat No. 14394-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Synonyms

AAC1, Arylamide acetylase 1, MNAT, N acetyltransferase type 1, NAT 1, NAT1, NATI

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inA375 cells, PC-3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA375 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14394-1-AP targets NAT1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NAT1 fusion protein Ag5885 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name N-acetyltransferase 1 (arylamine N-acetyltransferase)
Synonyms AAC1, Arylamide acetylase 1, MNAT, N acetyltransferase type 1, NAT 1, NAT1, NATI
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 32-34 kDa
GenBank Accession NumberBC047666
Gene Symbol NAT1
Gene ID (NCBI) 9
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP18440
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.