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NAT1 Polyclonal antibody

NAT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 19188-1-AP

Print datasheet

Synonyms

Monomorphic arylamine N-acetyltransferase, MNAT, EC:2.3.1.5, Arylamine N-acetyltransferase 1, Arylamide acetylase 1

验证数据展示

  • WB analysis of PC-3 using 19188-1-AP

    WB analysis of PC-3 using 19188-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 19188-1-AP (NAT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 19188-1-AP

    IHC staining of human breast cancer using 19188-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 19188-1-AP (NAT1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human breast cancer using 19188-1-AP

    IHC staining of human breast cancer using 19188-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 19188-1-AP (NAT1 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HeLa using 19188-1-AP

    IF Staining of HeLa using 19188-1-AP

    Immunofluorescent analysis of Hela cells, using NAT1 antibody 19188-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • WB analysis of PC-3 using 19188-1-AP

    WB analysis of PC-3 using 19188-1-AP

    WB result of NAT1 antibody (19188-1-AP; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAT1 transfected PC-3 cells.

经过测试的应用

Positive WB detected inPC-3 cells, human placenta tissue, A375 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19188-1-AP targets NAT1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen NAT1 fusion protein Ag6280 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name N-acetyltransferase 1 (arylamine N-acetyltransferase)
Synonyms Monomorphic arylamine N-acetyltransferase, MNAT, EC:2.3.1.5, Arylamine N-acetyltransferase 1, Arylamide acetylase 1
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 32-34 kDa
GenBank Accession NumberBC047666
Gene Symbol NAT1
Gene ID (NCBI) 9
RRIDAB_10641045
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP18440
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NAT1 antibody 19188-1-APDownload protocol
IHC protocol for NAT1 antibody 19188-1-APDownload protocol
IF protocol for NAT1 antibody 19188-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Oncol

Prognostic and Clinicopathological Significance of X-Box-Binding Protein 1 and N-Acetyltransferase 1 in Gallbladder Cancer.

Authors - Rushi Liu
View Article
humanWB,CoIP

Nucleic Acids Res

Integrated multi-omics approach revealed cellular senescence landscape

Authors - Qiao Song
View Article