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NAT13 Polyclonal antibody

NAT13 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16120-1-AP

Print datasheet

Synonyms

MAK3, hSAN, hNAT5, hNaa50p, EC:2.3.1.258

验证数据展示

  • WB analysis of K-562 using 16120-1-AP

    WB analysis of K-562 using 16120-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 16120-1-AP (NAT13 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 16120-1-AP

    IHC staining of human ovary cancer using 16120-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16120-1-AP (NAT13 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inK-562 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16120-1-AP targets NAT13 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NAT13 fusion protein Ag9075 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name N-acetyltransferase 13 (GCN5-related)
Synonyms MAK3, hSAN, hNAT5, hNaa50p, EC:2.3.1.258
Calculated Molecular Weight 169 aa, 19 kDa
Observed Molecular Weight 19 kDa
GenBank Accession NumberBC012731
Gene Symbol NAT13
Gene ID (NCBI) 80218
RRIDAB_2148952
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9GZZ1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NAT13 antibody 16120-1-APDownload protocol
IHC protocol for NAT13 antibody 16120-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols