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NAT5 Polyclonal antibody

NAT5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15807-1-AP

Print datasheet

Synonyms

N-alpha-acetyltransferase 20, N-acetyltransferase 5, NAA20, N acetyltransferase 5, Methionine N-acetyltransferase

验证数据展示

  • WB analysis of HeLa using 15807-1-AP

    WB analysis of HeLa using 15807-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15807-1-AP (NAT5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HL-60 using 15807-1-AP

    IP experiment of HL-60 using 15807-1-AP

    IP result of anti-NAT5 (IP:15807-1-AP, 4ug; Detection:15807-1-AP 1:500) with HL-60 cells lysate 3040ug.

  • IF Staining of HeLa using 15807-1-AP

    IF Staining of HeLa using 15807-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 15807-1-AP (NAT5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human ovary cancer using 15807-1-AP

    IHC staining of human ovary cancer using 15807-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 15807-1-AP (NAT5 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NAT5 antibody included

经过测试的应用

Positive WB detected inHeLa cells, HL-60 cells, human brain tissue
Positive IP detected inHL-60 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NAT5 Ready-To-Use IHC Kit. NAT5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

15807-1-AP targets NAT5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen NAT5 fusion protein Ag8541 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name N-acetyltransferase 5 (GCN5-related, putative)
Synonyms N-alpha-acetyltransferase 20, N-acetyltransferase 5, NAA20, N acetyltransferase 5, Methionine N-acetyltransferase
Calculated Molecular Weight 178 aa, 20 kDa
Observed Molecular Weight 18-20 kDa
GenBank Accession NumberBC005181
Gene Symbol NAT5
Gene ID (NCBI) 51126
RRIDAB_1640056
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61599
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NAT5 antibody 15807-1-APDownload protocol
IHC protocol for NAT5 antibody 15807-1-APDownload protocol
IF protocol for NAT5 antibody 15807-1-APDownload protocol
IP protocol for NAT5 antibody 15807-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Int J Mol Sci

NatB Catalytic Subunit Depletion Disrupts DNA Replication Initiation Leading to Senescence in MEFs

Authors - Jasmin Elurbide
View Article
humanWB

Oncotarget

NatB-mediated protein N-α-terminal acetylation is a potential therapeutic target in hepatocellular carcinoma.

Authors - Leire Neri
View Article
mouse

Mol Cell Biol

NatB Protects Procaspase-8 from UBR4-Mediated Degradation and Is Required for Full Induction of the Extrinsic Apoptosis Pathway

Authors - Joana P Guedes
View Article