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验证数据展示

  • IF Staining of HepG2 using 26150-1-AP

    IF Staining of HepG2 using 26150-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NBCe2 antibody (26150-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human liver cancer using 26150-1-AP

    IHC staining of human liver cancer using 26150-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26150-1-AP (NBCe2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NBCe2 Polyclonal antibody

NBCe2 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 26150-1-AP

Print datasheet

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (2)

Applications

IHC, IF/ICC, ELISA and More (1)

Synonyms

NBC4, NBCe2, SLC4A5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IHC detected inhuman liver cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

26150-1-AP targets NBCe2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, Mouse
Cited Reactivityhuman, mouse
Immunogen NBCe2 fusion protein Ag23558 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 4, sodium bicarbonate cotransporter, member 5
Synonyms NBC4, NBCe2, SLC4A5
Observed Molecular Weight108-127 kDa
GenBank Accession NumberBC109221
Gene Symbol SLC4A5
Gene ID (NCBI) 57835
RRIDAB_2918099
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BY07
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NBCe2, also named as SLC4A5 and NBC4, belongs to the anion exchanger (TC 2.A.31) family. NBCe2 mediates electrogenic Na+:HCO3 − transport, and can operate in both a 1:2, and 1:3 manner, but the transport stoichiometry as well as directionality have not been defined for the renal NBCe2. The expression of NBCe2 has been reported in nearly all kidney nephron segments and CDs, indicating that some species-specificity might be present (PMID: 32547422). NBCe2 has 8 isoforms with the molecular mass of 108-127 kDa.

实验方案

Product Specific Protocols
IHC protocol for NBCe2 antibody 26150-1-APDownload protocol
IF protocol for NBCe2 antibody 26150-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Mol Sci

Cell-Type Dependent Regulation of the Electrogenic Na+/HCO3- Cotransporter 1 (NBCe1) by Hypoxia and Acidosis in Glioblastoma

Authors - Marina Giannaki
View Article
mouseWB

Neuron

The sodium-bicarbonate cotransporter Slc4a5 mediates feedback at the first synapse of vision

Authors - Rei Morikawa
View Article