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验证数据展示

  • WB analysis of mouse colon using 12069-1-AP

    WB analysis of mouse colon using 12069-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 12069-1-AP (NBL1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

NBL1 Polyclonal antibody

NBL1 Polyclonal Antibody for WB, ELISA
Cat No. 12069-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

D1S1733E, DAN, DAN domain family member 1, DAND1, NB, NBL1, NO3, Protein N03, Zinc finger protein DAN

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse colon tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12069-1-AP targets NBL1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NBL1 fusion protein Ag2706 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neuroblastoma, suppression of tumorigenicity 1
别名 D1S1733E, DAN, DAN domain family member 1, DAND1, NB, NBL1, NO3, Protein N03, Zinc finger protein DAN
计算分子量 180 aa, 19 kDa
观测分子量 19 kDa
GenBank蛋白编号BC012037
基因名称 NBL1
Gene ID (NCBI) 4681
RRIDAB_10638604
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP41271
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NBL1 (neuroblastoma suppression of tumorigenicity 1), also named as DAN, is the founding member of the DAN subfamily. It was originally identified as a putative tumor suppressor gene in a transformed fibroblast rat model (PMID: 9516839), whereby it was significantly down-regulated in v-src-transformed rat fibroblasts. The MW of this protein is 19 kDa, and this antibody specially recognises the 19 kDa protein.

实验方案

Product Specific Protocols
WB protocol for NBL1 antibody 12069-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols