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NCAM1/CD56 Polyclonal antibody

NCAM1/CD56 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat No : 31536-1-AP

Print datasheet

Synonyms

CD56, Ncam1, NCAM 1, Ncam, E NCAM

验证数据展示

  • WB analysis using 31536-1-AP

    WB analysis using 31536-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31536-1-AP (NCAM1 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 31536-1-AP

    IHC staining of mouse brain using 31536-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31536-1-AP (NCAM1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 31536-1-AP

    IHC staining of mouse brain using 31536-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31536-1-AP (NCAM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31536-1-AP targets NCAM1/CD56 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity mouse, rat
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name neural cell adhesion molecule 1
Synonyms CD56, Ncam1, NCAM 1, Ncam, E NCAM
Calculated Molecular Weight119 kDa
Observed Molecular Weight120 kDa, 140 kDa, 180 kDa
Gene Symbol Ncam1
Gene ID (NCBI) 17967
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP13595-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isofroms have also been described (PMID: 1856291).

实验方案

Product Specific Protocols
WB protocol for NCAM1/CD56 antibody 31536-1-APDownload protocol
IHC protocol for NCAM1/CD56 antibody 31536-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols