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NCAM1/CD56 Recombinant antibody, PBS Only

NCAM1/CD56 Uni-rAbTM Recombinant Antibody for IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5N5

Cat no : 82715-3-PBS

Print datasheet

Synonyms

CD56, NCAM1, 5N5, NCAM 1, NCAM

验证数据展示

  • IHC staining of human tonsillitis using 82715-3-RR (same clone as 82715-3-PBS)

    IHC staining of human tonsillitis using 82715-3-RR (same clone as 82715-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82715-3-RR (NCAM1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82715-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD56/NCAM1 antibody included
  • IHC staining of human tonsillitis using 82715-3-RR (same clone as 82715-3-PBS)

    IHC staining of human tonsillitis using 82715-3-RR (same clone as 82715-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82715-3-RR (NCAM1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82715-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD56/NCAM1 antibody included
  • IF Staining of human tonsillitis using 82715-3-RR (same clone as 82715-3-PBS)

    IF Staining of human tonsillitis using 82715-3-RR (same clone as 82715-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using NCAM1/CD56 antibody (82715-3-RR, Clone: 5N5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82715-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82715-3-RR

    Affinity and Kinetic Characterization of 82715-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82715-3-RR against Human NCAM1/CD56 were performed. The affinity constant is 1.45 nM.

Planning an IHC experiment? We recommend our IHCeasy CD56/NCAM1 Ready-To-Use IHC Kit. CD56/NCAM1 primary antibody included.

产品信息

82715-3-PBS targets NCAM1/CD56 in IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity human
Immunogen NCAM1/CD56 fusion protein Ag5528 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name neural cell adhesion molecule 1
Synonyms CD56, NCAM1, 5N5, NCAM 1, NCAM
Calculated Molecular Weight 95 kDa
GenBank Accession NumberBC047244
Gene Symbol NCAM1
Gene ID (NCBI) 4684
ENSEMBL Gene IDENSG00000149294
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13591
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isoforms have also been described (PMID: 1856291).