NCAM2 Polyclonal antibody

NCAM2 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 13850-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA and More (1)

NCAM21, NCAM-2, NCAM 2, N CAM 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13850-1-AP targets NCAM2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 NCAM2 fusion protein Ag4821 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neural cell adhesion molecule 2
别名 NCAM21, NCAM-2, NCAM 2, N CAM 2
计算分子量 93 kDa
观测分子量 100-125 kDa
GenBank蛋白编号BC052946
基因名称 NCAM2
Gene ID (NCBI) 4685
RRIDAB_2237029
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15394
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The mammalian NCAM family has two members, NCAM1 and NCAM2 (PMID: 9729879; 20439718). Neural cell adhesion molecule 2 (NCAM2, also known as NCAM21) is a single-pass type I membrane protein that is highly expressed in the brain, which may play important roles in selective fasciculation and zone-to-zone projection of the primary olfactory axons or in neurodegenerative diseases such as Alzheimer's disease (PMID: 26611261). NCAM2 has two isoforms: NCAM2.1 bearing transmembrane and cytoplasmic domains and the shorter NCAM2.2 isoform, which is GPI-anchored (PMID: 9334170; 9221781). The molecular weight of NCAM2 is around 125 kDa and 93 kDa (PMID: 9334170; 3171685).

实验方案

Product Specific Protocols
WB protocol for NCAM2 antibody 13850-1-APDownload protocol
IHC protocol for NCAM2 antibody 13850-1-APDownload protocol
IP protocol for NCAM2 antibody 13850-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Science

Breast cancer exploits neural signaling pathways for bone-to-meninges metastasis

Authors - Andrew E Whiteley

J Comp Neurol

Illuminating the terminal nerve: Uncovering the link between GnRH-1 neuron and olfactory development

Authors - Enrico Amato
mouseIHC

bioRxiv

Illuminating the Terminal Nerve: Uncovering the Link between GnRH-1 and Olfactory Development

Authors - Enrico Amato
mouseIF

iScience

Single-nucleus RNA sequencing-based construction of a hippocampal neuron atlas in mice with epileptic cognitive impairment

Authors - Jia-Qi Ma
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