验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, human brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 2 publications below |
| IF | See 1 publications below |
产品信息
13850-1-AP targets NCAM2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IP, ELISA Application Description |
| Cited Applications | IHC, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Immunogen | NCAM2 fusion protein Ag4821 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | neural cell adhesion molecule 2 |
| Synonyms | NCAM21, NCAM-2, NCAM 2, N CAM 2 |
| Calculated Molecular Weight | 93 kDa |
| Observed Molecular Weight | 100-125 kDa |
| GenBank Accession Number | BC052946 |
| Gene Symbol | NCAM2 |
| Gene ID (NCBI) | 4685 |
| RRID | AB_2237029 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15394 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The mammalian NCAM family has two members, NCAM1 and NCAM2 (PMID: 9729879; 20439718). Neural cell adhesion molecule 2 (NCAM2, also known as NCAM21) is a single-pass type I membrane protein that is highly expressed in the brain, which may play important roles in selective fasciculation and zone-to-zone projection of the primary olfactory axons or in neurodegenerative diseases such as Alzheimer's disease (PMID: 26611261). NCAM2 has two isoforms: NCAM2.1 bearing transmembrane and cytoplasmic domains and the shorter NCAM2.2 isoform, which is GPI-anchored (PMID: 9334170; 9221781). The molecular weight of NCAM2 is around 125 kDa and 93 kDa (PMID: 9334170; 3171685).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NCAM2 antibody 13850-1-AP | Download protocol |
| IHC protocol for NCAM2 antibody 13850-1-AP | Download protocol |
| IP protocol for NCAM2 antibody 13850-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Comp Neurol Illuminating the terminal nerve: Uncovering the link between GnRH-1 neuron and olfactory development | ||
bioRxiv Illuminating the Terminal Nerve: Uncovering the Link between GnRH-1 and Olfactory Development | ||
iScience Single-nucleus RNA sequencing-based construction of a hippocampal neuron atlas in mice with epileptic cognitive impairment |
