验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, human brain tissue, rat brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | mouse brain tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 2 publications below |
IF | See 1 publications below |
产品信息
13850-1-AP targets NCAM2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IP, ELISA Application Description |
文献引用应用 | IHC, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | mouse |
免疫原 | NCAM2 fusion protein Ag4821 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | neural cell adhesion molecule 2 |
别名 | NCAM21, NCAM-2, NCAM 2, N CAM 2 |
计算分子量 | 93 kDa |
观测分子量 | 100-125 kDa |
GenBank蛋白编号 | BC052946 |
基因名称 | NCAM2 |
Gene ID (NCBI) | 4685 |
RRID | AB_2237029 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | O15394 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The mammalian NCAM family has two members, NCAM1 and NCAM2 (PMID: 9729879; 20439718). Neural cell adhesion molecule 2 (NCAM2, also known as NCAM21) is a single-pass type I membrane protein that is highly expressed in the brain, which may play important roles in selective fasciculation and zone-to-zone projection of the primary olfactory axons or in neurodegenerative diseases such as Alzheimer's disease (PMID: 26611261). NCAM2 has two isoforms: NCAM2.1 bearing transmembrane and cytoplasmic domains and the shorter NCAM2.2 isoform, which is GPI-anchored (PMID: 9334170; 9221781). The molecular weight of NCAM2 is around 125 kDa and 93 kDa (PMID: 9334170; 3171685).
实验方案
Product Specific Protocols | |
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WB protocol for NCAM2 antibody 13850-1-AP | Download protocol |
IHC protocol for NCAM2 antibody 13850-1-AP | Download protocol |
IP protocol for NCAM2 antibody 13850-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Comp Neurol Illuminating the terminal nerve: Uncovering the link between GnRH-1 neuron and olfactory development | ||
bioRxiv Illuminating the Terminal Nerve: Uncovering the Link between GnRH-1 and Olfactory Development | ||
iScience Single-nucleus RNA sequencing-based construction of a hippocampal neuron atlas in mice with epileptic cognitive impairment |