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NCAM2 Monoclonal antibody

NCAM2 Monoclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rabbit, Mouse, Rat

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1D6C12

Cat no : 68205-1-Ig

Print datasheet

Synonyms

N CAM 2, NCAM 2, NCAM2, NCAM21

验证数据展示

  • WB analysis using 68205-1-Ig

    WB analysis using 68205-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68205-1-Ig (NCAM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 68205-1-Ig

    IF Staining of mouse brain using 68205-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NCAM2 antibody (68205-1-Ig, Clone: 1D6C12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of mouse brain using 68205-1-Ig

    IF Staining of mouse brain using 68205-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NCAM2 antibody (68205-1-Ig, Clone: 1D6C12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inrabbit brain tissue, LNCaP cells, rat brain tissue, mouse brain tissue, rat cerebellum tissue, mouse cerebellum tissue
Positive IF-P detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68205-1-Ig targets NCAM2 in WB, IF-P, ELISA applications and shows reactivity with Human, Rabbit, Mouse, Rat samples.

Tested Applications WB, IF-P, ELISA Application Description
Tested Reactivity Human, Rabbit, Mouse, Rat
Immunogen NCAM2 fusion protein Ag4935 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name neural cell adhesion molecule 2
Synonyms N CAM 2, NCAM 2, NCAM2, NCAM21
Calculated Molecular Weight 93 kDa
Observed Molecular Weight93 kDa, 125 kDa
GenBank Accession NumberBC052946
Gene Symbol NCAM2
Gene ID (NCBI) 4685
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15394
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NCAM2 antibody 68205-1-IgDownload protocol
IF protocol for NCAM2 antibody 68205-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols