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验证数据展示

  • WB analysis using 68205-1-Ig

    WB analysis using 68205-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68205-1-Ig (NCAM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 68205-1-Ig

    IF Staining of mouse brain using 68205-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NCAM2 antibody (68205-1-Ig, Clone: 1D6C12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of mouse brain using 68205-1-Ig

    IF Staining of mouse brain using 68205-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NCAM2 antibody (68205-1-Ig, Clone: 1D6C12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

NCAM2 Monoclonal antibody

NCAM2 Monoclonal Antibody for WB, IF-P, ELISA
Cat No. 68205-1-Ig

产品说明书

CloneNo. 1D6C12

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Rabbit, Mouse, Rat

应用

WB, IF-P, ELISA

别名

N CAM 2, NCAM 2, NCAM2, NCAM21

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrabbit brain tissue, LNCaP cells, rat brain tissue, mouse brain tissue, rat cerebellum tissue, mouse cerebellum tissue
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68205-1-Ig targets NCAM2 in WB, IF-P, ELISA applications and shows reactivity with Human, Rabbit, Mouse, Rat samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 Human, Rabbit, Mouse, Rat
免疫原 NCAM2 fusion protein Ag4935 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 neural cell adhesion molecule 2
别名 N CAM 2, NCAM 2, NCAM2, NCAM21
计算分子量 93 kDa
观测分子量93 kDa, 125 kDa
GenBank蛋白编号BC052946
基因名称 NCAM2
Gene ID (NCBI) 4685
RRIDAB_2935293
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO15394
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NCAM2 antibody 68205-1-IgDownload protocol
IF protocol for NCAM2 antibody 68205-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols