验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells, MCF-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16828-1-AP targets NCAPD3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, bovine |
| 免疫原 | NCAPD3 fusion protein Ag10429 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | non-SMC condensin II complex, subunit D3 |
| 别名 | CAP D3, CAPD3, CNDD3, Condensin 2 complex subunit D3, hCAP D3, hCAP-D3, hcp 6, hHCP 6, KIAA0056, NCAPD3 |
| 计算分子量 | 1498 aa, 169 kDa |
| 观测分子量 | 165 kDa |
| GenBank蛋白编号 | BC011408 |
| 基因名称 | NCAPD3 |
| Gene ID (NCBI) | 23310 |
| RRID | AB_2282528 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P42695 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Condensin complexes I and II play essential roles in mitotic chromosome assembly and segregation. Both condensins contain 2 invariant structural maintenance of chromosome (SMC) subunits, SMC2 and SMC4 , but they contain different sets of non-SMC subunits. NCAPD3 is 1 of 3 non-SMC subunits that define condensin II ([PMID: 14532007).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NCAPD3 antibody 16828-1-AP | Download protocol |
| IF protocol for NCAPD3 antibody 16828-1-AP | Download protocol |
| IP protocol for NCAPD3 antibody 16828-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife Molecular dissection of condensin II-mediated chromosome assembly using in vitro assays | ||
Int J Mol Sci NCAPG Dynamically Coordinates the Myogenesis of Fetal Bovine Tissue by Adjusting Chromatin Accessibility. | ||
World J Gastroenterol Non-SMC condensin I complex subunit D2 and non-SMC condensin II complex subunit D3 induces inflammation via the IKK/NF-κB pathway in ulcerative colitis. | ||
Mol Cells NCAPH Stabilizes GEN1 in Chromatin to Resolve Ultra-Fine DNA Bridges and Maintain Chromosome Stability | ||
Histol Histopathol NCAPD3 is a prognostic biomarker and is correlated with immune infiltrates in glioma |
