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NCAPG Polyclonal antibody

NCAPG Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29933-1-AP

Print datasheet

Synonyms

CAPG, CHCG, Condensin complex subunit 3, Condensin subunit CAP G, FLJ12450, HCAP G, Melanoma antigen NY MEL 3, NCAPG, NY MEL 3, NYMEL3, XCAP G homolog

验证数据展示

  • WB analysis using 29933-1-AP

    WB analysis using 29933-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29933-1-AP (NCAPG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 29933-1-AP

    IHC staining of human ovary tumor using 29933-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29933-1-AP (NCAPG antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CAPG antibody included

经过测试的应用

Positive WB detected inK-562 cells, HEK-293 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CAPG Ready-To-Use IHC Kit. CAPG primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29933-1-AP targets NCAPG in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen NCAPG fusion protein Ag32040 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name non-SMC condensin I complex, subunit G
Synonyms CAPG, CHCG, Condensin complex subunit 3, Condensin subunit CAP G, FLJ12450, HCAP G, Melanoma antigen NY MEL 3, NCAPG, NY MEL 3, NYMEL3, XCAP G homolog
Calculated Molecular Weight 1015 aa, 114 kDa
Observed Molecular Weight131 kDa
GenBank Accession NumberBC068467
Gene Symbol NCAPG
Gene ID (NCBI) 64151
RRIDAB_2923621
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BPX3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NCAPG antibody 29933-1-APDownload protocol
IHC protocol for NCAPG antibody 29933-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols