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验证数据展示

  • WB analysis using 29933-1-AP

    WB analysis using 29933-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29933-1-AP (NCAPG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 29933-1-AP

    IHC staining of human ovary tumor using 29933-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29933-1-AP (NCAPG antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CAPG antibody included

NCAPG Polyclonal antibody

NCAPG Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29933-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IHC, ELISA

别名

CAPG, CHCG, Condensin complex subunit 3, Condensin subunit CAP G, FLJ12450, HCAP G, Melanoma antigen NY MEL 3, NCAPG, NY MEL 3, NYMEL3, XCAP G homolog

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, HEK-293 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CAPG Ready-To-Use IHC Kit. CAPG primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29933-1-AP targets NCAPG in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原 NCAPG fusion protein Ag32040 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 non-SMC condensin I complex, subunit G
别名 CAPG, CHCG, Condensin complex subunit 3, Condensin subunit CAP G, FLJ12450, HCAP G, Melanoma antigen NY MEL 3, NCAPG, NY MEL 3, NYMEL3, XCAP G homolog
计算分子量 1015 aa, 114 kDa
观测分子量131 kDa
GenBank蛋白编号BC068467
基因名称 NCAPG
Gene ID (NCBI) 64151
RRIDAB_2923621
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BPX3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NCAPG antibody 29933-1-APDownload protocol
IHC protocol for NCAPG antibody 29933-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols