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验证数据展示

  • WB analysis using 67655-1-Ig (same clone as 67655-1-PBS)

    WB analysis using 67655-1-Ig (same clone as 67655-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67655-1-Ig (NCAPH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67655-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67655-1-Ig (same clone as 67655-1-PBS)

    IHC staining of human liver cancer using 67655-1-Ig (same clone as 67655-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67655-1-Ig (NCAPH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67655-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NCAPH antibody included
  • IF Staining of human liver cancer using 67655-1-Ig (same clone as 67655-1-PBS)

    IF Staining of human liver cancer using 67655-1-Ig (same clone as 67655-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using NCAPH antibody (67655-1-Ig, Clone: 3D2F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67655-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 67655-1-Ig (same clone as 67655-1-PBS)

    WB analysis of HEK-293 using 67655-1-Ig (same clone as 67655-1-PBS)

    WB result of NCAPH antibody (67655-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NCAPH transfected HEK-293 cells. This data was developed using the same antibody clone with 67655-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

NCAPH Monoclonal antibody, PBS Only

NCAPH Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67655-1-PBS

产品说明书

CloneNo. 3D2F11

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

Barren homolog protein 1, BRRN, BRRN1, CAP H, CAPH, Condensin complex subunit 2, HCAP H, KIAA0074, NCAPH, XCAP H homolog

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy NCAPH Ready-To-Use IHC Kit. NCAPH primary antibody included.

产品信息

67655-1-PBS targets NCAPH in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 NCAPH fusion protein Ag27748 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 non-SMC condensin I complex, subunit H
别名 Barren homolog protein 1, BRRN, BRRN1, CAP H, CAPH, Condensin complex subunit 2, HCAP H, KIAA0074, NCAPH, XCAP H homolog
计算分子量 741 aa, 83 kDa
观测分子量 83-100 kDa
GenBank蛋白编号BC024211
基因名称 NCAPH
Gene ID (NCBI) 23397
RRIDAB_2918495
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15003
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Non-SMC condensin I complex subunit H (NCAPH) is one of the three non-SMC subunits in condensin I, which belongs to a recently defined superfamily of proteins termed kleisins. Another two non-SMC subunits, CAP-D2 and CAP-G, share a highly degenerate repeating motif known as HEAT repeat. Some studies show that each subunit is essential for viability and plays an important role in mitotic chromosome architecture and segregation. In recent years, researchers found that the high expression of NCAPH was associated with poor prognosis in patients with non-small cell lung cancer and prostate cancer. Downregulation of NCAPH inhibited the proliferation, migration, and invasion of several cancer cells significantly. Moreover, NCAPH was involved in the regulation of mature chromosome condensation and DNA damage. These data suggest that NCAPH may be a key carcinogen involved in the development and progression of human malignant tumors. ( PMID: 28300828, PMID: 33311486 )