验证数据展示
经过测试的应用
| Positive WB detected in | U-937 cells, Raji cells |
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28187-1-AP targets p47 phox in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | Human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | p47 phox fusion protein Ag28089 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | neutrophil cytosolic factor 1 |
| 别名 | FLJ79451, NCF 1, NCF 47K, NCF1, NCF1A, Neutrophil cytosol factor 1, neutrophil cytosolic factor 1, Nox organizer 2, Nox organizing protein 2, NOXO2, p47 phox, p47phox, p47-phox, SH3PXD1A |
| 计算分子量 | 45 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | BC002816 |
| 基因名称 | p47phox/NCF1 |
| Gene ID (NCBI) | 653361 |
| RRID | AB_2881084 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P14598 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for p47 phox antibody 28187-1-AP | Download protocol |
| IHC protocol for p47 phox antibody 28187-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Hazard Mater Graphene oxide disrupted mitochondrial homeostasis through inducing intracellular redox deviation and autophagy-lysosomal network dysfunction in SH-SY5Y cells. | ||
Oxid Med Cell Longev Human Tissue Kallikrein 1 Is Downregulated in Elderly Human Prostates and Possesses Potential In Vitro Antioxidative and Antifibrotic Effects in Rodent Prostates. | ||
Clin Nutr Chlorogenic acid protects against liver fibrosis in vivo and in vitro through inhibition of oxidative stress. | ||
Drug Des Devel Ther Artemisinin Improves Acetylcholine-Induced Vasodilatation in Rats with Primary Hypertension | ||
Lab Invest Pathological and biological significance of the specific glycan, TRA-1-60, on aggressive gastric adenocarcinoma
| ||
BMC Pulm Med Simvastatin attenuates silica-induced pulmonary inflammation and fibrosis in rats via the AMPK-NOX pathway |