验证数据展示
经过测试的应用
| Positive WB detected in | L02 cells |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
14648-1-AP targets NCF4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | rat |
| 免疫原 | NCF4 fusion protein Ag6241 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | neutrophil cytosolic factor 4, 40kDa |
| 别名 | P40PHOX, p40 phox, Neutrophil cytosol factor 4, NCF-4, NCF 4 |
| 计算分子量 | 39 kDa |
| 观测分子量 | 41 kDa |
| GenBank蛋白编号 | BC002798 |
| 基因名称 | NCF4 |
| Gene ID (NCBI) | 4689 |
| RRID | AB_2251237 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15080 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NCF4, also named as p40phox, was the last NADPH oxidase subunit to be discovered, and although there is evidence of its both positive and negative effects on oxidase function. Catalog#14648-1-AP is a rabbit polyclonal antibody raised against the full-length of human NCF4.The MW of this protein is 40 kDa, and this antibody specially recognises the 40 kDa protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NCF4 antibody 14648-1-AP | Download protocol |
| IHC protocol for NCF4 antibody 14648-1-AP | Download protocol |
| IF protocol for NCF4 antibody 14648-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
BMC Pulm Med Simvastatin attenuates silica-induced pulmonary inflammation and fibrosis in rats via the AMPK-NOX pathway | ||
CNS Neurosci Ther Neutrophil migration participates in the side effect of recombinant human tissue plasminogen activator |
