验证数据展示
经过测试的应用
| Positive WB detected in | L02 cells |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
14648-1-AP targets NCF4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | rat |
| Immunogen | NCF4 fusion protein Ag6241 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | neutrophil cytosolic factor 4, 40kDa |
| Synonyms | P40PHOX, p40 phox, Neutrophil cytosol factor 4, NCF-4, NCF 4 |
| Calculated Molecular Weight | 39 kDa |
| Observed Molecular Weight | 41 kDa |
| GenBank Accession Number | BC002798 |
| Gene Symbol | NCF4 |
| Gene ID (NCBI) | 4689 |
| RRID | AB_2251237 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15080 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NCF4, also named as p40phox, was the last NADPH oxidase subunit to be discovered, and although there is evidence of its both positive and negative effects on oxidase function. Catalog#14648-1-AP is a rabbit polyclonal antibody raised against the full-length of human NCF4.The MW of this protein is 40 kDa, and this antibody specially recognises the 40 kDa protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NCF4 antibody 14648-1-AP | Download protocol |
| IHC protocol for NCF4 antibody 14648-1-AP | Download protocol |
| IF protocol for NCF4 antibody 14648-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
BMC Pulm Med Simvastatin attenuates silica-induced pulmonary inflammation and fibrosis in rats via the AMPK-NOX pathway | ||
CNS Neurosci Ther Neutrophil migration participates in the side effect of recombinant human tissue plasminogen activator |
