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  • Featured Product
  • KD/KO Validated

NCK1 Polyclonal antibody

NCK1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat No : 15247-1-AP

Print datasheet

Synonyms

SH2/SH3 adapter protein NCK-alpha, SH2/SH3 adapter protein NCK1, NCKalpha, Nck-1, NCK alpha

验证数据展示

  • WB analysis of mouse brain using 15247-1-AP

    WB analysis of mouse brain using 15247-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15247-1-AP (NCK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 15247-1-AP

    WB analysis using 15247-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15247-1-AP (NCK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 15247-1-AP

    IHC staining of human breast cancer using 15247-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15247-1-AP (NCK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NCK1 antibody included
  • IHC staining of human breast cancer using 15247-1-AP

    IHC staining of human breast cancer using 15247-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15247-1-AP (NCK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NCK1 antibody included
  • IF Staining of HeLa using 15247-1-AP

    IF Staining of HeLa using 15247-1-AP

    Immunofluorescent analysis of Hela cells, using NCK1 antibody 15247-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of mouse brain using 15247-1-AP

    IP experiment of mouse brain using 15247-1-AP

    IP result of anti-NCK1 (IP:15247-1-AP, 4ug; Detection:15247-1-AP 1:500) with mouse brain tissue lysate 2640ug.

  • FC experiment of HeLa using 15247-1-AP

    FC experiment of HeLa using 15247-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug NCK1 Polyclonal antibody (15247-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inmouse brain tissue, mouse liver tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NCK1 Ready-To-Use IHC Kit. NCK1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15247-1-AP targets NCK1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen NCK1 fusion protein Ag0979 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NCK adaptor protein 1
Synonyms SH2/SH3 adapter protein NCK-alpha, SH2/SH3 adapter protein NCK1, NCKalpha, Nck-1, NCK alpha
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC006403
Gene Symbol NCK1
Gene ID (NCBI) 4690
RRIDAB_2150428
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP16333
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NCK family adaptor proteins function to couple tyrosine phosphorylation signals to regulate actin cytoskeletal reorganization that leads to cell motility. Cytoplasmic protein NCK1 (or NCK alpha) is an adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. NCK1 binds a number of intracellular proteins and influences various signaling pathways including GTPase-activating protein of Ras (RasGAP), which is responsible for the down-regulation of Ras. NCK1 is recently reported to regulate the UPR, which secondary to obesity impairs glucose homeostasis and INS actions.

实验方案

Product Specific Protocols
WB protocol for NCK1 antibody 15247-1-APDownload protocol
IHC protocol for NCK1 antibody 15247-1-APDownload protocol
IF protocol for NCK1 antibody 15247-1-APDownload protocol
IP protocol for NCK1 antibody 15247-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Signal

Identification of NCK1 as a novel downstream effector of STAT3 in colorectal cancer metastasis and angiogenesis.

Authors - Fan Zhang
  • KD Validated
View Article
humanWB,IP

Mol Immunol

The adapter proteins ADAP and Nck cooperate in T cell adhesion.

Authors - Marcus Lettau
  • KD Validated
View Article
humanWB,IHC

Mol Biol Rep

STAT3-induced NCK1 elevation promotes migration of triple-negative breast cancer cells via regulating ERK1/2 signaling

Authors - Peina He
  • KD Validated
View Article
humanWB

Cancer Biomark

Long noncoding RNA LINC00885 upregulates NCK1 to promote cell viability and migration of triple-negative breast cancer cells through sponging miR-654-3p

Authors - Peina He
View Article
humanWB

Cell Microbiol

The Legionella pneumophila effector WipA disrupts host F-actin polymerisation by hijacking phosphotyrosine signalling.

Authors - Lei He
View Article