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NCK2 Polyclonal antibody

NCK2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10206-1-AP

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Synonyms

Cytoplasmic protein NCK2, GRB4, Nck 2, NCK adaptor protein 2, NCK2, NCKbeta



经过测试的应用

Positive WB detected inU-937 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NCK2 Ready-To-Use IHC Kit. NCK2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10206-1-AP targets NCK2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen NCK2 fusion protein Ag0262 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NCK adaptor protein 2
Synonyms Cytoplasmic protein NCK2, GRB4, Nck 2, NCK adaptor protein 2, NCK2, NCKbeta
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC000103
Gene Symbol NCK2
Gene ID (NCBI) 8440
RRIDAB_2150571
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43639
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lots of the protein-protein interactions that are essential for eukaryotic intracellular signal transduction are mediated by protein binding modules including SH2, SH3, and LIM domains. NCK is a SH3- and SH2-containing adaptor protein implicated in coordinating various signaling pathways, including those of growth factor receptors and cell adhesion receptors. NCK-2 is a ubiquitously expressed and NCK-related adaptor protein comprising primarily three N-terminal SH3 domains and one C-terminal SH2 domain. NCK-2 interacts with PINCH, a LIM-only protein implicated in integrin-linked kinase signaling, and recognizes several key components of growth factor kinase-signaling pathways including EGF receptors, PDGF receptor, and IRS-1. NCK-2 may thus function as an adaptor protein connecting the growth factor receptor-signaling pathways with the integrin-signaling pathways. NCK-2 also interacts with focal adhesion kinase (FAK), a cytoplasmic protein tyrosine kinase critically involved in the cellular control of motility.

实验方案

Product Specific Protocols
WB protocol for NCK2 antibody 10206-1-APDownload protocol
IHC protocol for NCK2 antibody 10206-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Evid Based Complement Alternat Med

Icariin Alleviates Glucocorticoid-Induced Osteoporosis through EphB4/Ephrin-B2 Axis.

Authors - Mi Huang
humanWB

Biochem Pharmacol

KLF4 suppresses anticancer effects of brusatol via transcriptional upregulating NCK2 expression in melanoma

Authors - Xiaodong Li
  • KD Validated