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验证数据展示

  • WB analysis of mouse brain using 11367-1-AP

    WB analysis of mouse brain using 11367-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11367-1-AP (NCKIPSD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 11367-1-AP

    IP experiment of mouse brain using 11367-1-AP

    IP result of anti-NCKIPSD (IP:11367-1-AP, 4ug; Detection:11367-1-AP 1:300) with mouse brain tissue lysate 4000ug.

  • IHC staining of human breast cancer using 11367-1-AP

    IHC staining of human breast cancer using 11367-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11367-1-AP (NCKIPSD Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 11367-1-AP

    IHC staining of human breast cancer using 11367-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11367-1-AP (NCKIPSD Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

NCKIPSD Polyclonal antibody

NCKIPSD Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 11367-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, IHC, ELISA

别名

AF3P21, Dia interacting protein 1, DIP, DIP 1, DIP1, NCKIPSD, ORF1, SH3 adapter protein SPIN90, SPIN90, VIP54, WASLBP, WISH

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, fetal human brain tissue, human heart tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11367-1-AP targets NCKIPSD in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 NCKIPSD fusion protein Ag1915 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NCK interacting protein with SH3 domain
别名 AF3P21, Dia interacting protein 1, DIP, DIP 1, DIP1, NCKIPSD, ORF1, SH3 adapter protein SPIN90, SPIN90, VIP54, WASLBP, WISH
计算分子量 715 aa, 78 kDa
观测分子量 80 kDa
GenBank蛋白编号BC016052
基因名称 NCKIPSD
Gene ID (NCBI) 51517
RRIDAB_2150732
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NZQ3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NCKIPSD antibody 11367-1-APDownload protocol
IHC protocol for NCKIPSD antibody 11367-1-APDownload protocol
IP protocol for NCKIPSD antibody 11367-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC

Cancer Res

SPIN90 depletion and Microtubule Acetylation Mediate Stromal Fibroblast Activation in Breast Cancer Progression.

Authors - Eunae You
  • KO Validated
View Article