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  • Featured Product
  • KD/KO Validated

NCKIPSD Polyclonal antibody

NCKIPSD Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11367-1-AP

Print datasheet

Synonyms

AF3P21, Dia interacting protein 1, DIP, DIP 1, DIP1, NCKIPSD, ORF1, SH3 adapter protein SPIN90, SPIN90, VIP54, WASLBP, WISH

验证数据展示

  • WB analysis of mouse brain using 11367-1-AP

    WB analysis of mouse brain using 11367-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11367-1-AP (NCKIPSD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 11367-1-AP

    IP experiment of mouse brain using 11367-1-AP

    IP result of anti-NCKIPSD (IP:11367-1-AP, 4ug; Detection:11367-1-AP 1:300) with mouse brain tissue lysate 4000ug.

  • IHC staining of human breast cancer using 11367-1-AP

    IHC staining of human breast cancer using 11367-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11367-1-AP (NCKIPSD Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 11367-1-AP

    IHC staining of human breast cancer using 11367-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11367-1-AP (NCKIPSD Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, fetal human brain tissue, human heart tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11367-1-AP targets NCKIPSD in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen NCKIPSD fusion protein Ag1915 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NCK interacting protein with SH3 domain
Synonyms AF3P21, Dia interacting protein 1, DIP, DIP 1, DIP1, NCKIPSD, ORF1, SH3 adapter protein SPIN90, SPIN90, VIP54, WASLBP, WISH
Calculated Molecular Weight 715 aa, 78 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC016052
Gene Symbol NCKIPSD
Gene ID (NCBI) 51517
RRIDAB_2150732
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZQ3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NCKIPSD antibody 11367-1-APDownload protocol
IHC protocol for NCKIPSD antibody 11367-1-APDownload protocol
IP protocol for NCKIPSD antibody 11367-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC

Cancer Res

SPIN90 depletion and Microtubule Acetylation Mediate Stromal Fibroblast Activation in Breast Cancer Progression.

Authors - Eunae You
  • KO Validated
View Article