• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

NCOA1/SRC-1 Polyclonal antibody

NCOA1/SRC-1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 51114-1-AP

Print datasheet

Synonyms

bHLHe42, bHLHe74, F SRC 1, KAT13A, NCoA 1, NCOA1, nuclear receptor coactivator 1, Protein Hin 2, RIP160, SRC 1, SRC1, Steroid receptor coactivator 1

验证数据展示

  • WB analysis of mouse testis using 51114-1-AP

    WB analysis of mouse testis using 51114-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 51114-1-AP (NCOA1/SRC-1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 51114-1-AP

    IHC staining of human testis using 51114-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 51114-1-AP (NCOA1/SRC-1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 51114-1-AP

    IHC staining of human testis using 51114-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 51114-1-AP (NCOA1/SRC-1 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inmouse testis tissue, MCF-7 cells, PC-3 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

51114-1-AP targets NCOA1/SRC-1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nuclear receptor coactivator 1
Synonyms bHLHe42, bHLHe74, F SRC 1, KAT13A, NCoA 1, NCOA1, nuclear receptor coactivator 1, Protein Hin 2, RIP160, SRC 1, SRC1, Steroid receptor coactivator 1
Calculated Molecular Weight 1441 aa, 157 kDa
Observed Molecular Weight 140-160 kDa
GenBank Accession NumberBC111533
Gene Symbol SRC 1/NCOA1
Gene ID (NCBI) 8648
RRIDAB_2150905
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15788
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NCOA1/SRC-1 antibody 51114-1-APDownload protocol
IHC protocol for NCOA1/SRC-1 antibody 51114-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols