验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, HepG2 cells, Jurkat cells, LNCaP cells |
| Positive IF/ICC detected in | HepG2 cells, U2OS cells |
Planning an IHC experiment? We recommend our IHCeasy NCOA2 Ready-To-Use IHC Kit. NCOA2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
30962-1-AP targets NCOA2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | human |
| 文献引用反应性 | rat |
| 免疫原 | NCOA2 fusion protein Ag33768 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nuclear receptor coactivator 2 |
| 别名 | NCoA 2, KAT13C, hTIF2, GRIP1, Class E basic helix-loop-helix protein 75 |
| 计算分子量 | 1464 aa, 159 kDa |
| 观测分子量 | 160-180 kDa |
| GenBank蛋白编号 | BC114383 |
| 基因名称 | NCOA2 |
| Gene ID (NCBI) | 10499 |
| RRID | AB_3669796 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q15596 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NCOA2 antibody 30962-1-AP | Download protocol |
| IF protocol for NCOA2 antibody 30962-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |