验证数据展示
经过测试的应用
Positive WB detected in | Daudi cells, HEK-293 cells, Hela cells, Jurkat cells, K-562 cells, MCF-7 cells, PC-3 cells |
Positive IHC detected in | human lung cancer tissue, human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
29587-1-AP targets NCOA3 in WB, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, ELISA Application Description |
Tested Reactivity | human |
Immunogen | NCOA3 fusion protein Ag30633 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | nuclear receptor coactivator 3 |
Synonyms | AIB1, SRC3, CAGH 16, bHLHe42, AIB-1 |
Calculated Molecular Weight | 1424 aa, 155 kDa |
Observed Molecular Weight | 150-160 kDa |
GenBank Accession Number | BC119001 |
Gene Symbol | NCOA3 |
Gene ID (NCBI) | 8202 |
RRID | AB_2918328 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9Y6Q9 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NCOA3, also named as AIB1, SRC3 and TRAM1, belongs to the SRC/p160 nuclear receptor coactivator family. NCOA3 is a nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. NOCA3 plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). NCOA3 also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit.
实验方案
Product Specific Protocols | |
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WB protocol for NCOA3 antibody 29587-1-AP | Download protocol |
IHC protocol for NCOA3 antibody 29587-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |