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验证数据展示

  • WB analysis using 29587-1-AP

    WB analysis using 29587-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29587-1-AP (NCOA3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 29587-1-AP

    IHC staining of human lung cancer using 29587-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29587-1-AP (NCOA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 29587-1-AP

    WB analysis using 29587-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29587-1-AP (NCOA3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

NCOA3 Polyclonal antibody

NCOA3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29587-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

AIB1, SRC3, CAGH 16, bHLHe42, AIB-1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inDaudi cells, HEK-293 cells, Hela cells, Jurkat cells, K-562 cells, MCF-7 cells, PC-3 cells
Positive IHC detected inhuman lung cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29587-1-AP targets NCOA3 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 NCOA3 fusion protein Ag30633 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear receptor coactivator 3
别名 AIB1, SRC3, CAGH 16, bHLHe42, AIB-1
计算分子量 1424 aa, 155 kDa
观测分子量150-160 kDa
GenBank蛋白编号BC119001
基因名称 NCOA3
Gene ID (NCBI) 8202
RRIDAB_2918328
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6Q9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NCOA3, also named as AIB1, SRC3 and TRAM1, belongs to the SRC/p160 nuclear receptor coactivator family. NCOA3 is a nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. NOCA3 plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). NCOA3 also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit.

实验方案

Product Specific Protocols
WB protocol for NCOA3 antibody 29587-1-APDownload protocol
IHC protocol for NCOA3 antibody 29587-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols