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NCOA3 Polyclonal antibody, PBS Only

NCOA3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29587-1-PBS

Print datasheet

Synonyms

AIB1, SRC3, CAGH 16, bHLHe42, AIB-1

验证数据展示

  • WB analysis using 29587-1-AP (same clone as 29587-1-PBS)

    WB analysis using 29587-1-AP (same clone as 29587-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29587-1-AP (NCOA3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29587-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 29587-1-AP (same clone as 29587-1-PBS)

    IHC staining of human lung cancer using 29587-1-AP (same clone as 29587-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29587-1-AP (NCOA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29587-1-PBS in a different storage buffer formulation.

  • WB analysis using 29587-1-AP (same clone as 29587-1-PBS)

    WB analysis using 29587-1-AP (same clone as 29587-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29587-1-AP (NCOA3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29587-1-PBS in a different storage buffer formulation.

产品信息

29587-1-PBS targets NCOA3 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen NCOA3 fusion protein Ag30633 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nuclear receptor coactivator 3
Synonyms AIB1, SRC3, CAGH 16, bHLHe42, AIB-1
Calculated Molecular Weight 1424 aa, 155 kDa
Observed Molecular Weight150-160 kDa
GenBank Accession NumberBC119001
Gene Symbol NCOA3
Gene ID (NCBI) 8202
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6Q9
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NCOA3, also named as AIB1, SRC3 and TRAM1, belongs to the SRC/p160 nuclear receptor coactivator family. NCOA3 is a nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. NOCA3 plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). NCOA3 also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit.