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NCOA4 Recombinant antibody, PBS Only (Capture)

NCOA4 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240169H7

Cat no : 83394-4-PBS

Print datasheet

Synonyms

ELE1, ARA70, Androgen receptor-associated protein of 70 kDa, 70 kDa AR-activator, 70 kDa AR activator

验证数据展示

  • WB analysis using 83394-4-RR (same clone as 83394-4-PBS)

    WB analysis using 83394-4-RR (same clone as 83394-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83394-4-RR (NCOA4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83394-4-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83394-4-RR (same clone as 83394-4-PBS)

    IF Staining of HepG2 using 83394-4-RR (same clone as 83394-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NCOA4 antibody (83394-4-RR, Clone: 240169H7 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83394-4-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83394-4-RR (same clone as 83394-4-PBS)

    FC experiment of HeLa using 83394-4-RR (same clone as 83394-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug NCOA4 Recombinant antibody (83394-4-RR, Clone:240169H7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83394-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00435-1

    Cytometric bead array standard curve of MP00435-1

    Cytometric bead array standard curve of MP00435-1, NCOA4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83394-4-PBS. Detection antibody: 83394-2-PBS. Standard: Ag15277. Range: 3.13-100 ng/mL.

  • Affinity and Kinetic Characterization of 83394-4-RR

    Affinity and Kinetic Characterization of 83394-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83394-4-RR against Human NCOA4 were performed. The affinity constant is 0.586 nM.

产品信息

83394-4-PBS targets NCOA4 as part of a matched antibody pair:

MP00435-1: 83394-4-PBS capture and 83394-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen NCOA4 fusion protein Ag15277 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name nuclear receptor coactivator 4
Synonyms ELE1, ARA70, Androgen receptor-associated protein of 70 kDa, 70 kDa AR-activator, 70 kDa AR activator
Calculated Molecular Weight 70 kDa
GenBank Accession NumberBC012736
Gene Symbol NCOA4
Gene ID (NCBI) 8031
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13772
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C