验证数据展示
经过测试的应用
| Positive IP detected in | HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy NCOA6 Ready-To-Use IHC Kit. NCOA6 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25241-1-AP targets NCOA6 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | NCOA6 fusion protein Ag18737 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nuclear receptor coactivator 6 |
| 别名 | AIB3, ANTP, ASC 2, ASC2, HOX1.1, HOXA7, KIAA0181, NCOA6, NRC, NRC RAP250, nuclear receptor coactivator 6, PPAR interacting protein, PRIP, RAP250, TRBP |
| 计算分子量 | 2063 aa, 219 kDa |
| 观测分子量 | 68 kDa, 219 kDa, 110 kDa |
| GenBank蛋白编号 | BC136272 |
| 基因名称 | NCOA6 |
| Gene ID (NCBI) | 23054 |
| RRID | AB_2879983 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14686 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NCOA6 (Nuclear receptor coactivator 6), also named as AIB3 or ANTP, is a 2063 amino acid protein, which contains two LXXLL motifs. NCOA6 localizes in the nucleus and is highly expressed in brain, prostate, testis and ovary. NCOA6 directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. The molecular weight of NCOA6 is 219 kDa, but we always detected 68 kDa protein by western blot.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NCOA6 antibody 25241-1-AP | Download protocol |
| IP protocol for NCOA6 antibody 25241-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol TRACERx analysis identifies a role for FAT1 in regulating chromosomal instability and whole-genome doubling via Hippo signalling | ||
Mol Ther Nucleic Acids Downregulated lncRNA HOXA11-AS Affects Trophoblast Cell Proliferation and Migration by Regulating RND3 and HOXA7 Expression in PE. | ||
Mol Pharmacol Alterations of Histone Modifications Contribute to Pregnane X Receptor-mediated Induction of CYP3A4 by Rifampicin. | ||
Cancer Med Nuclear receptor coactivator 6 (NCoA6) promotes cell proliferation, migration, and invasion in pancreatic cancer
|
