验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, MCF-7 cells |
| Positive IP detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23092-1-AP targets NCOA7 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | NCOA7 fusion protein Ag19315 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nuclear receptor coactivator 7 |
| 别名 | ERAP140, ESNA1, Nbla00052, Nbla10993, NCOA7, nuclear receptor coactivator 7 |
| 计算分子量 | 972 aa, 106 kDa |
| 观测分子量 | 120-130 kDa |
| GenBank蛋白编号 | BC137094 |
| 基因名称 | NCOA7 |
| Gene ID (NCBI) | 135112 |
| RRID | AB_2879208 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8NI08 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nuclear receptors for steroids, thyroid hormones and retinoic acids are ligand-dependent transcription factors that activate transcription through specific DNA binding sites in their target genes. NCoA-7 (nuclear receptor coactivator 7), also known as ESNA1 or ERAP140, is a 942 amino acid nuclear protein that enhances nuclear receptor transcriptional activities and coactivates several nuclear receptors including PPARG, ESR1, THRB and RARA. Highly expressed in brain and weakly expressed in pancreas, bladder, ovary, spinal cord, prostate, mammary gland, ovary, uterus and stomach, NCoA-7 is a member of the OXR1 family and contains one LysM repeat and a TLD domain. This antibody is a rabbit polyclonal antibody raised against the N-terminal of human NCOA7 protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NCOA7 antibody 23092-1-AP | Download protocol |
| IP protocol for NCOA7 antibody 23092-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Mol Life Sci The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour.
| ||
Am J Physiol Renal Physiol Targeted Deletion of the Ncoa7 Gene Results in Incomplete Distal Renal Tubular Acidosis in Mice. | ||
Int J Mol Sci NCOA7 Regulates Growth and Metastasis of Clear Cell Renal Cell Carcinoma via MAPK/ERK Signaling Pathway |
