验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, MOLT-4 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Planning an IHC experiment? We recommend our IHCeasy NCOR2 Ready-To-Use IHC Kit. NCOR2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
29952-1-AP targets NCOR2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human |
| 文献引用反应性 | human |
| 免疫原 | NCOR2 fusion protein Ag30727 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nuclear receptor co-repressor 2 |
| 别名 | CTG repeat protein 26, CTG26, N CoR2, NCOR2, Nuclear receptor corepressor 2, SMAP270, SMRT, SMRTE, SMRTE tau, T3 receptor associating factor, TNRC14, TRAC, TRAC 1, TRAC1 |
| 计算分子量 | 274 kDa |
| 观测分子量 | 274 kDa |
| GenBank蛋白编号 | NM_006312 |
| 基因名称 | NCOR2 |
| Gene ID (NCBI) | 9612 |
| RRID | AB_3086197 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y618 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NCOR2, nuclear receptor corepressor 2 , is a transcriptional corepressor that that contains several nuclear receptor-interacting domains. NCOR2/SMRT acts as a platform protein, facilitating the recruitment of histone deacetylases to the DNA promoters bound by its interacting transcription factors (PMID: 9150137). NCOR2 mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription (PMID: 23081895).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NCOR2 antibody 29952-1-AP | Download protocol |
| IHC protocol for NCOR2 antibody 29952-1-AP | Download protocol |
| IF protocol for NCOR2 antibody 29952-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
