NCS1 Monoclonal antibody

NCS1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, IF-P, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

2F10C8

Cat no : 67616-1-Ig

Print datasheet

Synonyms

DKFZp761L1223, FLUP, FREQ, Frequenin homolog, frequenin homolog (Drosophila), Frequenin like protein, NCS 1, NCS1, Neuronal calcium sensor 1



经过测试的应用

Positive WB detected inU-251 cells, U-87 MG cells, pig brain tissue, pig cerebellum tissue, rat brain tissue, rat cerebellum tissue, mouse brain tissue, mouse cerebellum tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

67616-1-Ig targets NCS1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity Human, mouse, rat, pig
Cited Reactivitymouse
Immunogen NCS1 fusion protein Ag29808 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name frequenin homolog (Drosophila)
Synonyms DKFZp761L1223, FLUP, FREQ, Frequenin homolog, frequenin homolog (Drosophila), Frequenin like protein, NCS 1, NCS1, Neuronal calcium sensor 1
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC004856
Gene Symbol NCS1
Gene ID (NCBI) 23413
RRIDAB_2882819
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP62166
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NCS1 antibody 67616-1-IgDownload protocol
IHC protocol for NCS1 antibody 67616-1-IgDownload protocol
IF protocol for NCS1 antibody 67616-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Int J Mol Sci

Neuronal Calcium Sensor-1 Protects Cortical Neurons from Hyperexcitation and Ca2+ Overload during Ischemia by Protecting the Population of GABAergic Neurons

Authors - Elena G Varlamova