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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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NCX1 Polyclonal antibody

NCX1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (1)

Applications

WB, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 55075-1-AP

Print datasheet

Synonyms

验证数据展示

  • WB analysis of mouse brain using 55075-1-AP

    WB analysis of mouse brain using 55075-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55075-1-AP (NCX1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 55075-1-AP

    IP experiment of mouse brain using 55075-1-AP

    IP result of anti-NCX1 (IP:55075-1-AP, 4ug; Detection:55075-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy SLC8A1 Ready-To-Use IHC Kit. SLC8A1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55075-1-AP targets NCX1 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IP, CoIP
Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse, rat, chicken
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 8 (sodium/calcium exchanger), member 1
Synonyms
Calculated Molecular Weight 109 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberNM_021097
Gene Symbol NCX1
Gene ID (NCBI) 6546
RRIDAB_2881262
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP32418
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NCX1 antibody 55075-1-APDownload protocol
IP protocol for NCX1 antibody 55075-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC

J Am Soc Nephrol

Gentamicin Inhibits Ca2+ Channel TRPV5 and Induces Calciuresis Independent of the Calcium-Sensing Receptor-Claudin-14 Pathway.

Authors - Wouter H van Megen
View Article
ratWB

Front Immunol

Interleukin-6-Mediated-Ca2+ Handling Abnormalities Contributes to Atrial Fibrillation in Sterile Pericarditis Rats.

Authors - Jie Liao
View Article
mouseIP

J Am Heart Assoc

Sodium-Glucose Cotransporter 2 Inhibitor Canagliflozin Antagonizes Salt-Sensitive Hypertension Through Modifying Transient Receptor Potential Channels 3 Mediated Vascular Calcium Handling.

Authors - Yu Zhao
View Article
chickenWB

Chemosphere

NF-κB-mediated inflammation correlates with calcium overload under arsenic trioxide-induced myocardial damage in Gallus gallus.

Authors - Siwen Li
View Article
mouseWB

Alcohol Clin Exp Res

Impact and related mechanisms of chronic moderate alcohol consumption on cardiac function in aldehyde dehydrogenase 2 heterozygous mice.

Authors - Qinfeng Hu
View Article
ratCoIP

Front Physiol

PMID: 32009985

Authors - Raúl Sampieri
View Article