验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue |
| Positive IHC detected in | human stomach tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19704-1-AP targets ND2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | NADH dehydrogenase, subunit 2 (complex I) |
| 别名 | MT-ND2, ND 2, NADH2, NADH dehydrogenase subunit 2, MTND2 |
| 计算分子量 | 39 kDa |
| 观测分子量 | 42-44 kDa |
| GenBank蛋白编号 | GQ398480 |
| 基因名称 | ND2 |
| Gene ID (NCBI) | 4536 |
| RRID | AB_10638920 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P03891 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ND2, also named as MTND2, NADH2 and ND2, belongs to the complex I subunit 2 family. ND2 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ND2 antibody 19704-1-AP | Download protocol |
| IHC protocol for ND2 antibody 19704-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Med The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity. | ||
Mol Cell Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis | ||
Mol Cell Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.
| ||
Nat Chem Biol NSUN3 methylase initiates 5-formylcytidine biogenesis in human mitochondrial tRNA(Met). | ||
Nucleic Acids Res Hypertension-associated mitochondrial DNA 4401A>G mutation caused the aberrant processing of tRNAMet, all 8 tRNAs and ND6 mRNA in the light-strand transcript. |