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验证数据展示

  • IF Staining of HepG2 using 31132-1-AP

    IF Staining of HepG2 using 31132-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ND5 antibody (31132-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

MT-ND5 Polyclonal antibody

MT-ND5 Polyclonal Antibody for IF/ICC, ELISA
Cat No. 31132-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, ELISA

别名

ND5, ND 5, NADH5, MTND5, MT ND5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31132-1-AP targets MT-ND5 in IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 fusion protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NADH dehydrogenase, subunit 5 (complex I)
别名 ND5, ND 5, NADH5, MTND5, MT ND5
计算分子量67 kDa
GenBank蛋白编号YP_003024036
基因名称 MT-ND5
Gene ID (NCBI) 4540
RRIDAB_3669867
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP03915
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ND5 belongs to the complex I subunit 1 family. ND5 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

实验方案

Product Specific Protocols
IF protocol for MT-ND5 antibody 31132-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols