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验证数据展示

  • IF Staining of HepG2 using 31132-1-AP

    IF Staining of HepG2 using 31132-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ND5 antibody (31132-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

MT-ND5 Polyclonal antibody

MT-ND5 Polyclonal Antibody for IF/ICC, ELISA
Cat No. 31132-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

ND5, ND 5, NADH5, MTND5, MT ND5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31132-1-AP targets MT-ND5 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen fusion protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NADH dehydrogenase, subunit 5 (complex I)
Synonyms ND5, ND 5, NADH5, MTND5, MT ND5
Calculated Molecular Weight67 kDa
GenBank Accession NumberYP_003024036
Gene Symbol MT-ND5
Gene ID (NCBI) 4540
RRIDAB_3669867
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP03915
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ND5 belongs to the complex I subunit 1 family. ND5 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

实验方案

Product Specific Protocols
IF protocol for MT-ND5 antibody 31132-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols