MT-ND5 Polyclonal antibody

MT-ND5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 55410-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, ELISA

ND5, ND 5, NADH5, MTND5, MT ND5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 30 publications below

产品信息

55410-1-AP targets MT-ND5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, hamster
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NADH dehydrogenase, subunit 5 (complex I)
别名 ND5, ND 5, NADH5, MTND5, MT ND5
计算分子量 67 kDa
观测分子量 65-70 kDa
GenBank蛋白编号AF465942
基因名称 MT-ND5
Gene ID (NCBI) 4540
RRIDAB_2881324
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP03915
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ND5 belongs to the complex I subunit 1 family. ND5 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

实验方案

Product Specific Protocols
WB protocol for MT-ND5 antibody 55410-1-APDownload protocol
IHC protocol for MT-ND5 antibody 55410-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Metab

Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.

Authors - Yi-Ping Wang
humanWB

Mol Cell

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

Authors - Bin Jiang
humanWB

Sci Adv

MCART1/SLC25A51 is required for mitochondrial NAD transport.

Authors - Nora Kory
humanWB

EMBO J

EglN2 associates with the NRF1-PGC1α complex and controls mitochondrial function in breast cancer.

Authors - Jing Zhang
humanWB

EMBO Rep

MITRAC15/COA1 promotes mitochondrial translation in a ND2 ribosome-nascent chain complex.

Authors - Cong Wang
humanWB

Cell Mol Gastroenterol Hepatol

A Mitochondrial DNA Variant Elevates the Risk of Gallstone Disease by Altering Mitochondrial Function.

Authors - Dayan Sun
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