验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, NCCIT cells, HSC-T6 cells, LNCaP cells, Jurkat cells, THP-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66960-1-Ig targets NDC80 in WB, ELISA applications and shows reactivity with Human, rat, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, rat, mouse |
| 免疫原 | NDC80 fusion protein Ag8065 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | NDC80 homolog, kinetochore complex component (S. cerevisiae) |
| 别名 | HEC, HEC1, HsHec1, hsNDC80, Kinetochore protein Hec1, KNTC2, NDC80, TID3 |
| 计算分子量 | 74 kDa |
| 观测分子量 | 71-74 kDa |
| GenBank蛋白编号 | BC035617 |
| 基因名称 | NDC80 |
| Gene ID (NCBI) | 10403 |
| RRID | AB_2882283 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O14777 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NDC80 antibody 66960-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
