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NDE1 Recombinant antibody

NDE1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

242340H4

Cat no : 84799-4-RR

Print datasheet

Synonyms

NUDE1, NudE, HOM TES 87, 242340H4

验证数据展示

  • WB analysis using 84799-4-RR

    WB analysis using 84799-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84799-4-RR (NDE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 84799-4-RR

    IHC staining of mouse liver using 84799-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84799-4-RR (NDE1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 84799-4-RR

    IHC staining of mouse liver using 84799-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84799-4-RR (NDE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHepG2 cells, SKOV-3 cells, HEK-293 cells, human heart tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84799-4-RR targets NDE1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NDE1 fusion protein Ag0336 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name nudE nuclear distribution gene E homolog 1 (A. nidulans)
Synonyms NUDE1, NudE, HOM TES 87, 242340H4
Calculated Molecular Weight 335 aa, 38 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC001421
Gene Symbol NDE1
Gene ID (NCBI) 54820
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NXR1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NDE1 (NUDE /NUDE1) is a LIS1-interacting protein. A signaling pathway containing NDE1, LIS1, and cytoplasmic dynein is conserved for eukaryotic nuclear migration and has profound impact on neuronal migration during development of the central nervous system. LIS1 and dynein have been shown to be involved in mitosis and NDE1 participates in a subset of dynein function in M phase. NDE1 is specifically phosphorylated in M phase in its serine/threonine phosphorylation motifs, probably by Cdc2 and also Erk1 and Erk2. A fraction of NDEl binds to centrosomes strongly in interphase and is localized to mitotic spindles in early M phase. Phosphorylation of NDE1 also regulates the cell-cycle-dependent distribution, possibly by increasing its dissociation rate at the microtubule-organizing center.

实验方案

Product Specific Protocols
WB protocol for NDE1 antibody 84799-4-RRDownload protocol
IHC protocol for NDE1 antibody 84799-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols