验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, SH-SY5Y cells |
| Positive IP detected in | HEK-293 cells |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15602-1-AP targets NDFIP1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | NDFIP1 fusion protein Ag7985 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Nedd4 family interacting protein 1 |
| 别名 | PSEC0223, PSEC0192, NEDD4 WW domain-binding protein 5, NEDD4 family-interacting protein 1, N4WBP5 |
| 计算分子量 | 25 kDa |
| 观测分子量 | 25-30 kDa |
| GenBank蛋白编号 | BC004317 |
| 基因名称 | NDFIP1 |
| Gene ID (NCBI) | 80762 |
| RRID | AB_2878157 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BT67 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ndfip1 is an adaptor protein for the Nedd4 family of ubiquitin ligases and has been found to be upregulated in neurons after brain injury, including head trauma, stroke and metal toxicity. Recent studies confirmed the role of Ndfip1 as a regulator of iron metabolism and pointed out that Ndfip1 was involved in iron homeostasis by regulating the degradation of iron importer divalent metal transporter 1. Ndfip1 is a transmembrane protein that is localized to the Golgi and post-Golgi vesicles such as endosomes. It is an adaptor protein that recruits E3 ligases to ubiquitinate target proteins. It is ubiquitously expressed and contains 2 PY motifs, which interact with several Nedd4 family E3s to degradate proteins.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NDFIP1 antibody 15602-1-AP | Download protocol |
| IF protocol for NDFIP1 antibody 15602-1-AP | Download protocol |
| IP protocol for NDFIP1 antibody 15602-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell PKCλ/ι inhibition activates an ULK2-mediated interferon response to repress tumorigenesis. | ||
Cell Death Discov WWP1 upregulation predicts poor prognosis and promotes tumor progression by regulating ubiquitination of NDFIP1 in intrahepatic cholangiocarcinoma. | ||
Nat Chem Biol µMap proximity labeling in living cells reveals stress granule disassembly mechanisms
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