NDP Polyclonal antibody
NDP Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse testis tissue, mouse eye tissue |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 15380-1-AP is NDP Fusion Protein expressed in E. coli.
Tested Applications | WB, ELISA Application Description |
Tested Reactivity | human, mouse |
Immunogen | NDP fusion protein Ag4066 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | Norrie disease (pseudoglioma) |
Synonyms | EVR2, FEVR, ND, NDP, Norrie disease (pseudoglioma), Norrie disease protein, Norrin |
Calculated Molecular Weight | 15 kDa |
Observed Molecular Weight | 50-60 kDa |
GenBank Accession Number | BC029901 |
Gene Symbol | NDP |
Gene ID (NCBI) | 4693 |
RRID | AB_2878133 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q00604 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NDP, also named as EVR2, ND and Norrin, is a secreted regulatory protein that remains tightly associated with the extracellular matrix. Mutations in the NDP gene are associated with the Norrie disease. Signaling induced by the protein NDP regulates vascular development of vertebrate retina and controls important blood vessels in the ear. It binds with high affinity to Frizzled 4, and Frizzled 4 knockout mice exhibit abnormal vascular development of the retina. This antibody detects 11-15 kDa (monomer) and 48 kDa (polymers, refer: 25692504).
实验方案
Product Specific Protocols | |
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WB protocol for NDP antibody 15380-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |