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  • Featured Product
  • KD/KO Validated

NDP52 Monoclonal antibody, PBS Only

NDP52 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F2C2

Cat no : 66401-1-PBS

Print datasheet

Synonyms

CALCOCO2, Nuclear dot protein 52, Nuclear domain 10 protein 52, Calcium-binding and coiled-coil domain-containing protein 2, 2F2C2

验证数据展示

  • WB analysis using 66401-1-Ig (same clone as 66401-1-PBS)

    WB analysis using 66401-1-Ig (same clone as 66401-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66401-1-Ig (NDP52 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66401-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66401-1-Ig (same clone as 66401-1-PBS)

    WB analysis of HeLa using 66401-1-Ig (same clone as 66401-1-PBS)

    WB result of NDP52 antibody (66401-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDP52 transfected HeLa cells. This data was developed using the same antibody clone with 66401-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66401-1-Ig (same clone as 66401-1-PBS)

    WB analysis of HeLa using 66401-1-Ig (same clone as 66401-1-PBS)

    HeLa, Jurkat cells and human skeletal muscle tissue lysate were subjected to SDS PAGE followed by western blot with 66401-1-Ig (NDP52 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66401-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 66401-1-Ig (same clone as 66401-1-PBS)

    IHC staining of human ovary tumor using 66401-1-Ig (same clone as 66401-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66401-1-Ig (NDP52 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66401-1-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 66401-1-Ig (same clone as 66401-1-PBS)

    IF Staining of Jurkat using 66401-1-Ig (same clone as 66401-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using NDP52 antibody (66401-1-Ig, Clone: 2F2C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66401-1-PBS in a different storage buffer formulation.

产品信息

66401-1-PBS targets NDP52 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NDP52 fusion protein Ag2866 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name calcium binding and coiled-coil domain 2
Synonyms CALCOCO2, Nuclear dot protein 52, Nuclear domain 10 protein 52, Calcium-binding and coiled-coil domain-containing protein 2, 2F2C2
Calculated Molecular Weight 446 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC015893
Gene Symbol NDP52
Gene ID (NCBI) 10241
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13137
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NDP52, also named as CALCOCO2, is an autophagy receptor. It plays a role in ruffle formation and actin cytoskeleton organization. Mouse/Rat NDP52 has some isoforms with MW 28-40 kDa and 67 kDa. Human NDP52 has some isoforms with MW 43-47 kDa and 52-55 kDa,