• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

NDP52 Monoclonal antibody, PBS Only

NDP52 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F2C2

Cat no : 66401-1-PBS

Print datasheet

Synonyms

CALCOCO2, Nuclear dot protein 52, Nuclear domain 10 protein 52, Calcium-binding and coiled-coil domain-containing protein 2, 2F2C2

验证数据展示

  • WB analysis using 66401-1-Ig (same clone as 66401-1-PBS)

    WB analysis using 66401-1-Ig (same clone as 66401-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66401-1-Ig (NDP52 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66401-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66401-1-Ig (same clone as 66401-1-PBS)

    WB analysis of HeLa using 66401-1-Ig (same clone as 66401-1-PBS)

    WB result of NDP52 antibody (66401-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDP52 transfected HeLa cells. This data was developed using the same antibody clone with 66401-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66401-1-Ig (same clone as 66401-1-PBS)

    WB analysis of HeLa using 66401-1-Ig (same clone as 66401-1-PBS)

    HeLa, Jurkat cells and human skeletal muscle tissue lysate were subjected to SDS PAGE followed by western blot with 66401-1-Ig (NDP52 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66401-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 66401-1-Ig (same clone as 66401-1-PBS)

    IHC staining of human ovary tumor using 66401-1-Ig (same clone as 66401-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66401-1-Ig (NDP52 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66401-1-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 66401-1-Ig (same clone as 66401-1-PBS)

    IF Staining of Jurkat using 66401-1-Ig (same clone as 66401-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using NDP52 antibody (66401-1-Ig, Clone: 2F2C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66401-1-PBS in a different storage buffer formulation.

产品信息

66401-1-PBS targets NDP52 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NDP52 fusion protein Ag2866 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name calcium binding and coiled-coil domain 2
Synonyms CALCOCO2, Nuclear dot protein 52, Nuclear domain 10 protein 52, Calcium-binding and coiled-coil domain-containing protein 2, 2F2C2
Calculated Molecular Weight 446 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC015893
Gene Symbol NDP52
Gene ID (NCBI) 10241
RRIDAB_2881775
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13137
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NDP52, also named as CALCOCO2, is an autophagy receptor. It plays a role in ruffle formation and actin cytoskeleton organization. Mouse/Rat NDP52 has some isoforms with MW 28-40 kDa and 67 kDa. Human NDP52 has some isoforms with MW 43-47 kDa and 52-55 kDa,