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NDRG2 Polyclonal antibody

NDRG2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12015-1-AP

Print datasheet

Synonyms

KIAA1248, NDRG family member 2, NDRG2, Protein NDRG2, Protein Syld709613, SYLD

验证数据展示

  • IHC staining of mouse brain using 12015-1-AP

    IHC staining of mouse brain using 12015-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12015-1-AP (NDRG2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NDRG2 antibody included
  • IHC staining of mouse brain using 12015-1-AP

    IHC staining of mouse brain using 12015-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12015-1-AP (NDRG2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NDRG2 antibody included
  • WB analysis of mouse brain using 12015-1-AP

    WB analysis of mouse brain using 12015-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12015-1-AP (NDRG2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of rat brain using 12015-1-AP

    IP experiment of rat brain using 12015-1-AP

    IP result of anti-NDRG2 (IP:12015-1-AP, 4ug; Detection:12015-1-AP 1:1000) with rat brain tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inrat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NDRG2 Ready-To-Use IHC Kit. NDRG2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12015-1-AP targets NDRG2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen NDRG2 fusion protein Ag2629 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NDRG family member 2
Synonyms KIAA1248, NDRG family member 2, NDRG2, Protein NDRG2, Protein Syld709613, SYLD
Calculated Molecular Weight 371 aa, 39 kDa
Observed Molecular Weight 41-43 kDa
GenBank Accession NumberBC010458
Gene Symbol NDRG2
Gene ID (NCBI) 57447
RRIDAB_10666856
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UN36
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NDRG2 antibody 12015-1-APDownload protocol
IHC protocol for NDRG2 antibody 12015-1-APDownload protocol
IP protocol for NDRG2 antibody 12015-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseIHC

Front Immunol

Single-cell protein activity analysis reveals a novel subpopulation of chondrocytes and the corresponding key master regulator proteins associated with anti-senescence and OA progression

Authors - Zhao Guang
View Article
humanWB

J Ovarian Res

NDRG2 gene expression pattern in ovarian cancer and its specific roles in inhibiting cancer cell proliferation and suppressing cancer cell apoptosis.

Authors - Fenhong Kang
View Article