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NDRG2 Monoclonal antibody

NDRG2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rat, Mouse, Pig and More (1)

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

3E3F4

Cat no : 67191-1-Ig

Print datasheet

Synonyms

DKFZp781G1938, FLJ25522, KIAA1248, NDRG family member 2, NDRG2, Protein NDRG2, Protein Syld709613, SYLD

验证数据展示

  • WB analysis using 67191-1-Ig

    WB analysis using 67191-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67191-1-Ig (NDRG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 67191-1-Ig

    IHC staining of mouse brain using 67191-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67191-1-Ig (NDRG2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NDRG2 antibody included
  • IHC staining of mouse brain using 67191-1-Ig

    IHC staining of mouse brain using 67191-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67191-1-Ig (NDRG2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NDRG2 antibody included

经过测试的应用

Positive WB detected inhuman heart tissue, pig brain tissue, rat brain tissue, mouse brain tissue, pig heart tissue, rat heart tissue
Positive IHC detected inmouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NDRG2 Ready-To-Use IHC Kit. NDRG2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

67191-1-Ig targets NDRG2 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Rat, Mouse, Pig samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity Human, Rat, Mouse, Pig
Cited Reactivitymouse
Immunogen NDRG2 fusion protein Ag28612 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name NDRG family member 2
Synonyms DKFZp781G1938, FLJ25522, KIAA1248, NDRG family member 2, NDRG2, Protein NDRG2, Protein Syld709613, SYLD
Calculated Molecular Weight 371 aa, 39 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC010458
Gene Symbol NDRG2
Gene ID (NCBI) 57447
RRIDAB_2882486
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UN36
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NDRG2 antibody 67191-1-IgDownload protocol
IHC protocol for NDRG2 antibody 67191-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Sci Adv

Astrocytic NDRG2-PPM1A interaction exacerbates blood-brain barrier disruption after subarachnoid hemorrhage

Authors - Dayun Feng
View Article