NDRG4 Polyclonal antibody
NDRG4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, mouse kidney tissue |
| Positive IHC detected in | human medulloblastoma tissue, human colon tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
12184-1-AP targets NDRG4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | NDRG4 fusion protein Ag3029 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | NDRG family member 4 |
| Synonyms | SMAP 8, Protein NDRG4, N-myc downstream-regulated gene 4 protein, NDRG family member 4, Brain development-related molecule 1 |
| Calculated Molecular Weight | 339 aa, 37 kDa |
| Observed Molecular Weight | 37 kDa, 38 kDa and 41 kDa |
| GenBank Accession Number | BC011795 |
| Gene Symbol | NDRG4 |
| Gene ID (NCBI) | 65009 |
| RRID | AB_2235852 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9ULP0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NDRG4 antibody 12184-1-AP | Download protocol |
| IHC protocol for NDRG4 antibody 12184-1-AP | Download protocol |
| IF protocol for NDRG4 antibody 12184-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
DNA Cell Biol The Role of N-myc Downstream-Regulated Gene Family in Glioma Based on Bioinformatics Analysis. | ||
BMB Rep Hypermethylation-mediated silencing of NDRG4 promotes pancreatic ductal adenocarcinoma by regulating mitochondrial function. | ||
Cell Rep The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids. |
