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验证数据展示

  • WB analysis using 15889-1-AP

    WB analysis using 15889-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15889-1-AP (NDUFA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 15889-1-AP

    IHC staining of mouse heart using 15889-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15889-1-AP (NDUFA2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 15889-1-AP

    IHC staining of mouse heart using 15889-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15889-1-AP (NDUFA2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NDUFA2 Polyclonal antibody

NDUFA2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15889-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

B8, CI B8, Complex I B8, NDUFA2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue, mouse liver tissue, rat heart tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15889-1-AP targets NDUFA2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NDUFA2 fusion protein Ag8675 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 2, 8kDa
别名 B8, CI B8, Complex I B8, NDUFA2
计算分子量 99 aa, 11 kDa
观测分子量 11 kDa
GenBank蛋白编号BC003674
基因名称 NDUFA2
Gene ID (NCBI) 4695
RRIDAB_3669223
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43678
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NDUFA2(NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2), also known as NADH ubiquinone oxidoreductase B8 subunit, is one of accessory subunits of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I). NDUFS2 is vital for growth, ROS generation, membrane integrity, apoptosis, and mitochondrial energetics.

实验方案

Product Specific Protocols
WB protocol for NDUFA2 antibody 15889-1-APDownload protocol
IHC protocol for NDUFA2 antibody 15889-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols