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NDUFA4L2 Recombinant antibody

NDUFA4L2 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241622B12

Cat no : 84498-1-RR

Print datasheet

Synonyms

NUOMS, NADH-ubiquinone oxidoreductase MLRQ subunit homolog, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4-like 2, 241622B12

验证数据展示

  • WB analysis using 84498-1-RR

    WB analysis using 84498-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84498-1-RR (NDUFA4L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 84498-1-RR

    IHC staining of mouse kidney using 84498-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84498-1-RR (NDUFA4L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NDUFA4L2 antibody included
  • IHC staining of mouse kidney using 84498-1-RR

    IHC staining of mouse kidney using 84498-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84498-1-RR (NDUFA4L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NDUFA4L2 antibody included
  • IF Staining of HeLa using 84498-1-RR

    IF Staining of HeLa using 84498-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NDUFA4L2 antibody (84498-1-RR, Clone: 241622B12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84498-1-RR

    Affinity and Kinetic Characterization of 84498-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84498-1-RR against Human NDUFA4L2 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, PC-3 cells, human liver tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NDUFA4L2 Ready-To-Use IHC Kit. NDUFA4L2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84498-1-RR targets NDUFA4L2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen NDUFA4L2 fusion protein Ag9600 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4-like 2
Synonyms NUOMS, NADH-ubiquinone oxidoreductase MLRQ subunit homolog, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4-like 2, 241622B12
Calculated Molecular Weight 87 aa, 10 kDa
Observed Molecular Weight 10 kDa
GenBank Accession NumberBC011910
Gene Symbol NDUFA4L2
Gene ID (NCBI) 56901
RRIDAB_3672011
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9NRX3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NDUFA4L2 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4-like 2), also named as NUOMS, is a HIF-1α target gene that localizes to the mitochondria and belongs to the complex I NDUFA4 subunit family. It downregulates oxygen consumption and Complex I activity in hypoxia. NDUFA4L2 is involved in hypoxic adaptation by decreasing mitochondrial ROS production.

实验方案

Product Specific Protocols
WB protocol for NDUFA4L2 antibody 84498-1-RRDownload protocol
IHC protocol for NDUFA4L2 antibody 84498-1-RRDownload protocol
IF protocol for NDUFA4L2 antibody 84498-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols