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NDUFA4L2 Recombinant antibody

NDUFA4L2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241622B12

Cat no : 84498-1-RR

Print datasheet

Synonyms

NUOMS, NADH-ubiquinone oxidoreductase MLRQ subunit homolog, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4-like 2, 241622B12

验证数据展示

  • WB analysis using 84498-1-RR

    WB analysis using 84498-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84498-1-RR (NDUFA4L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 84498-1-RR

    IHC staining of mouse kidney using 84498-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84498-1-RR (NDUFA4L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NDUFA4L2 antibody included
  • IHC staining of mouse kidney using 84498-1-RR

    IHC staining of mouse kidney using 84498-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84498-1-RR (NDUFA4L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NDUFA4L2 antibody included
  • IF Staining of HeLa using 84498-1-RR

    IF Staining of HeLa using 84498-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NDUFA4L2 antibody (84498-1-RR, Clone: 241622B12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84498-1-RR

    Affinity and Kinetic Characterization of 84498-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84498-1-RR against Human NDUFA4L2 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, PC-3 cells, human liver tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NDUFA4L2 Ready-To-Use IHC Kit. NDUFA4L2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84498-1-RR is NDUFA4L2 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen NDUFA4L2 fusion protein Ag9600 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4-like 2
Synonyms NUOMS, NADH-ubiquinone oxidoreductase MLRQ subunit homolog, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4-like 2, 241622B12
Calculated Molecular Weight 87 aa, 10 kDa
Observed Molecular Weight 10 kDa
GenBank Accession NumberBC011910
Gene Symbol NDUFA4L2
Gene ID (NCBI) 56901
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9NRX3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NDUFA4L2 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4-like 2), also named as NUOMS, is a HIF-1α target gene that localizes to the mitochondria and belongs to the complex I NDUFA4 subunit family. It downregulates oxygen consumption and Complex I activity in hypoxia. NDUFA4L2 is involved in hypoxic adaptation by decreasing mitochondrial ROS production.

实验方案

Product Specific Protocols
WB protocol for NDUFA4L2 antibody 84498-1-RRDownload protocol
IHC protocol for NDUFA4L2 antibody 84498-1-RRDownload protocol
IF protocol for NDUFA4L2 antibody 84498-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols