验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse brain tissue, HepG2 cells, HEK-293 cells, rat brain tissue, mouse heart tissue |
Planning an IHC experiment? We recommend our IHCeasy NDUFA5 Ready-To-Use IHC Kit. NDUFA5 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85187-1-RR targets NDUFA5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | NDUFA5 fusion protein Ag9995 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa |
| 别名 | Complex I subunit B13, Complex I 13kD B, CI-13kD-B, CI 13KD B, B13 |
| 计算分子量 | 13 kDa |
| 观测分子量 | 13 kDa |
| GenBank蛋白编号 | BC000813 |
| 基因名称 | NDUFA5 |
| Gene ID (NCBI) | 4698 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q16718 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NDUFA5, also named as CI-13kD-B and NDUA5, belongs to the complex I NDUFA5 subunit family. It is accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which functions in the transfer of electrons from NADH to the respiratory chain. NDUFA5,an enrichment of mitochondrial protein, can be used as a mitochondrial marker protein(PMID:21360670).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NDUFA5 antibody 85187-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |