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NDUFAF1 Polyclonal antibody

NDUFAF1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15181-1-AP

Print datasheet

Synonyms

Complex I intermediate-associated protein 30, mitochondrial, CIA30, CGI-65, CGI65, CGI 65

验证数据展示

  • WB analysis of HEK-293 using 15181-1-AP

    WB analysis of HEK-293 using 15181-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15181-1-AP (NDUFAF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human oesophagus cancer using 15181-1-AP

    IHC staining of human oesophagus cancer using 15181-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15181-1-AP (NDUFAF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NDUFAF1 antibody included
  • IHC staining of human oesophagus cancer using 15181-1-AP

    IHC staining of human oesophagus cancer using 15181-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15181-1-AP (NDUFAF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NDUFAF1 antibody included

经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, Transfected HEK-293 cells, HeLa cells
Positive IHC detected inhuman oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NDUFAF1 Ready-To-Use IHC Kit. NDUFAF1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

15181-1-AP targets NDUFAF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen NDUFAF1 fusion protein Ag7162 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 1
Synonyms Complex I intermediate-associated protein 30, mitochondrial, CIA30, CGI-65, CGI65, CGI 65
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC000780
Gene Symbol NDUFAF1
Gene ID (NCBI) 51103
RRIDAB_2150778
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y375
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NDUFAF1 antibody 15181-1-APDownload protocol
IHC protocol for NDUFAF1 antibody 15181-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Stem Cell Res Ther

Mitochondrial activity regulates the differentiation of skin-derived mesenchymal stem cells into brown adipocytes to contribute to hypertension.

Authors - Wenda Xi
View Article
mouseWB

Sci Rep

Integrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by microRNAs.

Authors - David W Reid
View Article