• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

NDUFB2 Polyclonal antibody

NDUFB2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17614-1-AP

Print datasheet

Synonyms

NADH-ubiquinone oxidoreductase AGGG subunit, Complex I-AGGG, Complex I AGGG, CI-AGGG, CI AGGG

验证数据展示

  • WB analysis using 17614-1-AP

    WB analysis using 17614-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17614-1-AP (NDUFB2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 17614-1-AP

    IHC staining of mouse brain using 17614-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17614-1-AP (NDUFB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 17614-1-AP

    IHC staining of mouse brain using 17614-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17614-1-AP (NDUFB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of C2C12 using 17614-1-AP

    IF Staining of C2C12 using 17614-1-AP

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using NDUFB2 antibody (17614-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHEK-293T cells, mouse spleen tissue, human heart tissue, mouse heart tissue, rat heart tissue, HuH-7 cells, MCF-7 cells, mouse brain tissue, mouse kidney tissue
Positive IHC detected inmouse brain tissue, human heart tissue, human kidney tissue, human skin tissue, human liver tissue, human ovary tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inC2C12 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17614-1-AP targets NDUFB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen NDUFB2 fusion protein Ag11789 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 2, 8kDa
Synonyms NADH-ubiquinone oxidoreductase AGGG subunit, Complex I-AGGG, Complex I AGGG, CI-AGGG, CI AGGG
Calculated Molecular Weight 95 aa, 11 kDa
Observed Molecular Weight 8-12 kDa
GenBank Accession NumberBC063026
Gene Symbol NDUFB2
Gene ID (NCBI) 4708
RRIDAB_2150944
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95178
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The multisubunit NADH:ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of mitochondria. NDUFB2, also named as CI-AGGG, is a nuclear encoded subunit located in the hydrophobic protein fraction of complex I(PMID:9878551). It may have a role in the regulation of molecular changes during the global cardiac ischemia/reperfusion injury during cardiopulmonary bypass (CPB). The full length 12 kDa protein has a transit peptide with 33 amino acids.

实验方案

Product Specific Protocols
WB protocol for NDUFB2 antibody 17614-1-APDownload protocol
IHC protocol for NDUFB2 antibody 17614-1-APDownload protocol
IF protocol for NDUFB2 antibody 17614-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Immunol

Identification and validation of cuproptosis-related genes in acetaminophen-induced liver injury using bioinformatics analysis and machine learning

Authors - Zhenya Guo
View Article