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NDUFB3 Polyclonal antibody

NDUFB3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12358-1-AP

Print datasheet

Synonyms

B12, CI B12, Complex I B12, NDUFB3

验证数据展示

  • WB analysis of human heart using 12358-1-AP

    WB analysis of human heart using 12358-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 12358-1-AP (NDUFB3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 12358-1-AP

    IHC staining of human liver cancer using 12358-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12358-1-AP (NDUFB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 12358-1-AP

    IHC staining of human liver cancer using 12358-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12358-1-AP (NDUFB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman heart tissue, mouse heart tissue, rat heart tissue
Positive IHC detected inhuman liver cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12358-1-AP targets NDUFB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen NDUFB3 fusion protein Ag3022 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 3, 12kDa
Synonyms B12, CI B12, Complex I B12, NDUFB3
Calculated Molecular Weight 98 aa, 11 kDa
Observed Molecular Weight 11 kDa
GenBank Accession NumberBC018183
Gene Symbol NDUFB3
Gene ID (NCBI) 4709
RRIDAB_2282633
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43676
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NDUFB3(NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3) is also named as CI-B12 and belongs to the complex I NDUFB3 subunit family.It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis.

实验方案

Product Specific Protocols
WB protocol for NDUFB3 antibody 12358-1-APDownload protocol
IHC protocol for NDUFB3 antibody 12358-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Hazard Mater

Maternal exposure to E 551 during pregnancy leads to genome-wide DNA methylation changes and metabolic disorders in the livers of pregnant mice and their fetuses

Authors - Yingqi Zhan
View Article