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NDUFB5 Polyclonal antibody

NDUFB5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23855-1-AP

Print datasheet

Synonyms

CI SGDH, Complex I SGDH, NDUFB5, SGDH

验证数据展示

  • WB analysis of mouse liver using 23855-1-AP

    WB analysis of mouse liver using 23855-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 23855-1-AP (NDUFB5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 23855-1-AP

    IHC staining of human liver cancer using 23855-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23855-1-AP (NDUFB5 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver cancer using 23855-1-AP

    IHC staining of human liver cancer using 23855-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23855-1-AP (NDUFB5 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inmouse liver tissue, mouse spleen tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

23855-1-AP targets NDUFB5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Immunogen NDUFB5 fusion protein Ag20907 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 5, 16kDa
Synonyms CI SGDH, Complex I SGDH, NDUFB5, SGDH
Calculated Molecular Weight 189 aa, 22 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC005271
Gene Symbol NDUFB5
Gene ID (NCBI) 4711
RRIDAB_2879342
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDO43674
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NDUFB5 antibody 23855-1-APDownload protocol
IHC protocol for NDUFB5 antibody 23855-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Proteome Sci

Effect of downregulated citrate synthase on oxidative phosphorylation signaling pathway in HEI-OC1 cells

Authors - Xiaowen Xu
View Article
ratWB

Basic Res Cardiol

Comparison of the stage-dependent mitochondrial changes in response to pressure overload between the diseased right and left ventricle in the rat

Authors - Ling Li
View Article